Complication of Anesthesia
Changes in expression of hippocampal long non-coding RNA and bioinformatics analysis in mice with perioperative neurocognitive disorders
Li Xiaoxiao, Wang Bin, Dong Rui, Yu Jingfu, Zhou Jiahui, Lin Xu, Wang Mingshan, Bi Yanlin
Published 2019-03-20
Cite as Chin J Anesthesiol, 2019,39(3): 304-308. DOI: 10.3760/cma.j.issn.0254-1416.2019.03.014
Abstract
ObjectiveTo evaluate the changes in the expression of hippocampal long non-coding RNA and bioinformatics analysis in mice with perioperative neurocognitive disorders (PND).
MethodsThirty clean-grade male C57BL/6 mice, aged 12-14 weeks, weighing 25-30 g, were divided into 2 groups (n=15 each) according to the random number table method: control group (group C) and PND group.The model of PND was established by performing open tibial fracture with intramedullary fixation under isoflurane anesthesia in anesthetized mice.The Morris water maze test, open field test and fear conditioning test were performed at 1, 3 and 7 days postoperatively.The animals were sacrificed after the end of behavioral testing on 3 days after operation, the hippocampus was obtained, the high-throughput gene sequencing was performed to identify the differentially expressed lncRNAs, and Gene Ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes signaling pathway analysis were used to analyze the results.
ResultsCompared with group C, the escape latency was significantly prolonged, and the percentage of time spend in the target quadrant and percentage of freezing time in the fear conditioning test were decreased at different time points after operation in group PND(P<0.05). A total of 121 differentially expressed lncRNAs were identified, of which 69 were up-regulated and 52 were down-regulated.The Gene Ontology functional analysis showed that there were differences in various biological processes, such as synaptic transmission, cholinergic neurotransmitters, or adiponectin secretion and regulation.The KEGG signaling pathway analysis showed that there were also differences in cholinergic synapses, MAPK signaling pathway, glucagon signaling pathway, TNF signaling pathway, NOD-like receptors, Toll-like receptors, chemokine signaling pathway and etc.
ConclusionThere are 121 differentially expressed lncRNAs in the hippocampus of PND mice, and lncRNAs- and the target gene-related inflammatory responses, synaptic transmission, energy metabolism and etc.may be related to the pathogenesis of PND.
Key words:
Cognition disorders; Hippocampus; Long non-coding RNA
Contributor Information
Li Xiaoxiao
Department of Anesthesiology, Weifang Medical College, Weifang 261053, China
Wang Bin
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China
Dong Rui
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China
Yu Jingfu
Department of Anesthesiology, Affiliated Hospital of Qingdao University, Qingdao 266000, China
Zhou Jiahui
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China
Lin Xu
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China
Wang Mingshan
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China
Bi Yanlin
Department of Anesthesiology, East Hospital of Qingdao Municipal Hospital, Qingdao 266000, China