Critical Care Medicine
Role of HDAC3 in renal injury induced by myocardial ischemia-reperfusion in diabetic rats
Hao Ming, Zhen Qiu, Zhongyuan Xia, Shaoqing Lei, Bo Zhao
Published 2019-08-20
Cite as Chin J Anesthesiol, 2019, 39(8): 993-995. DOI: 10.3760/cma.j.issn.0254-1416.2019.08.024
Abstract
ObjectiveTo evaluate the role of histone deacetylase 3 (HDAC3) in renal injury induced by myocardial ischemia-reperfusion (I/R) in diabetic rats.
MethodsSPF healthy adult male Sprague-Dawley rats, weighing 210-220 g, were used in this study.Diabetes mellitus was induced by intraperitoneal injection of 1% streptozotocin 60 mg/kg.Eighteen diabetic rats were divided into 3 groups (n=6 each) using a random number table method: sham operation group (group S), group I/R and HDAC3 inhibitor RGFP966 group (RG). The myocardial I/R model was established by ligation of the left anterior descending coronary artery for 30 min followed by 120-min reperfusion.RGFP966 10 mg/kg was intraperitoneally injected at 1 h before myocardial ischemia in group RG.The right internal carotid artery was isolated at 120 min of reperfusion for measurement of serum lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), creatinine (Cr) and urea nitrogen concentrations.Renal tissues were obtained for examination of the pathological changes and for determination of the expression of HDAC3, silent information regulator 1 (SIRT1) and interleukin-1β (IL-1β). The damage to the renal tubules was scored.
ResultsCompared with S group, the serum LDH, CK-MB, Cr and urea nitrogen concentrations and renal tubular damage score were significantly increased, the expression of HDAC3 and IL-1β was up-regulated, and the expression of SIRT1 was down-regulated in group I/R (P<0.05). Compared with group I/R, the serum LDH, CK-MB, Cr and urea nitrogen concentrations and renal tubular damage score were significantly decreased, the expression of HDAC3 and IL-1β was down-regulated, and the expression of SIRT1 was up-regulated in group RG (P<0.05).
ConclusionUp-regulated expression of HDAC3 is involved in renal injury induced by myocardial I/R in diabetic rats.
Key words:
Histone deacetylases; Diabetes mellitus; Myocardial reperfusion injury; Acute kidney injury
Contributor Information
Hao Ming
Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Zhen Qiu
Zhongyuan Xia
Shaoqing Lei
Bo Zhao