Critical Care Medicine
Role of microRNA-125b in ventilator-induced lung injury in mice
Tianfeng Huang, Ju Gao, Luojing Zhou, Yali Ge, Cunjin Wang
Published 2019-09-20
Cite as Chin J Anesthesiol, 2019, 39(9): 1121-1124. DOI: 10.3760/cma.j.issn.0254-1416.2019.09.024
Abstract
ObjectiveTo evaluate the role of microRNA-125b (miR-125b) on ventilator-induced lung injury (VILI) in mice.
MethodsForty healthy male C57BL/6 male mice, weighing 25-30 g, aged 2-3 months, were divided into 4 groups (n=10 each) using a random number table method: sham operation group (group Sham), group VILI, VILI plus miR-125b negative control group (group VILI+ NC), and VILI plus miR-125b overexpression group (group VILI+ miR-125b agomir). In VILI+ NC and VILI+ miR-125b agomir groups, miR-125b negative control and miR-125b agomir transfection complex 50 μl were intratracheally instilled, respectively, and 48 h later VILI model was established.The animals were mechanically ventilated for 4 h with high tidal volume (40 ml/kg) to induce VILI.Blood samples were obtained from the femoral artery at 4 h of mechanical ventilation for detection of PaO2, then animals were sacrificed, lungs were removed for determination of wet to dry weight ratio (W/D ratio) and for examination of pathological changes (with a light microscope), and lung injury was scored.In VILI+ NC and VILI+ miR-125b agomir groups, bronchoalveolar lavage fluid (BALF) was collected to measure the concentrations of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by enzyme-linked immunosorbent assay.The cell apoptosis of lung tissues was measured using TUNEL, apoptosis index was calculated, the caspase-3 expression was detected by Western blot, and the miR-125b expression was detected by real-time polymerase chain reaction.
ResultsCompared with Sham group, PaO2 was significantly decreased, and W/D ratio and lung injury score were increased in VILI, VILI+ NC and VILI+ miR-125b agomir groups, and the expression of miR-125b was down-regulated in VILI and VILI+ NC groups (P<0.05). Compared with VILI group, PaO2 was significantly increased, W/D ratio and lung injury score were decreased, the expression of miR-125b was up-regulated (P<0.05), the pathological changes of lung tissues were significantly attenuated in group VILI+ miR-125b agomir, and no significant change was found in the parameters mentioned above in group VILI+ NC (P<0.05). Compared with group VILI+ NC, the concentrations of TNF-α and IL-6 in BALF and apoptotic index were significantly decreased, and the expression of caspase-3 was down regulated in group VILI+ miR-125b agomir (P<0.05).
ConclusionMiR-125b is involved in the endogenous protective mechanism of VILI in mice.
Key words:
MicroRNAs; Ventilator-induced lung injury
Contributor Information
Tianfeng Huang
Department of Anesthesiology, Institute of Anesthesiology and Critical Care, Northern Jiangsu People′s Hospital Affiliated to Yangzhou University, Yangzhou 225001, China
Ju Gao
Luojing Zhou
Yali Ge
Cunjin Wang