Complication of Anesthesia
Role of hippocampal CD200R1 in perioperative neurocognitive disorders in mice
Ma Danxu, Liu Jinhu, Shen Wenzhen, Wei Changwei, Xiong Chao, Lin Dandan, Xue Ziyi, Wu Anshi
Published 2019-10-20
Cite as Chin J Anesthesiol, 2019,39(10): 1181-1184. DOI: 10.3760/cma.j.issn.0254-1416.2019.10.009
Abstract
ObjectiveTo evaluate the role of hippocampal CD200 receptor 1 (CD200R1) in perioperative neurocognitive disorders (PND) in mice.
MethodsSixty clean-grade male C57BL/6 mice, aged 9-10 months, weighing 32-38 g, were used in the study.The experiment was performed in two parts.Experiment Ⅰ Thirty-six mice were divided into 2 groups (n=18 each) using a random number table method: control group (group C) and PND group.Group C only received isoflurane anesthesia.Partial left lobectomy of the liver was performed under isoflurane anesthesia in group PND.Contextual fear conditioning test was performed at 1, 3 and 7 days after surgery, and the freezing time was recorded.The mice were then sacrificed, and the hippocampus was isolated for determination of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents (by enzyme-linked immunosorbent assay) and CD200 and CD200R1 expression (by Western blot). Experiment Ⅱ Twenty-four mice were divided into 2 groups (n=12 each) using a random number table method: CD200-Fc group and IgG1-Fc group.Recombinant proteins CD200-Fc and human IgG1-Fc were injected into the lateral cerebral ventricle in CD200-Fc group and IgG1-Fc group, respectively.Partial left lobectomy of the liver was performed after the end of injection.Contextual fear conditioning test was performed at 1 and 3 days after surgery, and the freezing time was recorded.
ResultsExperiment Ⅰ Compared with group C, the freezing time in the contextual fear conditioning test was significantly shortened, and the contents of IL-1β were increased at 1 and 3 days after surgery, the contents of TNF-α were increased at 3 and 7 days after surgery, and the expression of CD200 and CD200R1 was up-regulated at 1 day after surgery in group PND (P<0.05). Experiment Ⅱ Compared with IgG1-Fc group, the freezing time in the contextual fear conditioning test was significantly prolonged at 1 day after surgery in CD200-Fc group (P<0.05).
ConclusionUp-regulated expression of hippocampal CD200R1 is the endogenous protective mechanism of PND in mice.
Key words:
Neurocognitive disorders; CD200R1
Contributor Information
Ma Danxu
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Liu Jinhu
Department of Anesthesiology, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing 100026, China
Shen Wenzhen
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Wei Changwei
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Xiong Chao
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Lin Dandan
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Xue Ziyi
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Wu Anshi
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
