Experimental Researche
Physical-chemical properties and enhancement effect of Integrin αvβ3/CCR2 dual targeted ultrasound microbubbles: a vitro study
Yingying Liu, Jinfeng Xu, Fei Yan, Hui Luo, Xinxin Zeng, Zhanghong Wei, Huiyu Liu, Yuli Zhou
Published 2015-05-25
Cite as Chin J Ultrasonogr, 2015, 24(5): 440-444. DOI: 10.3760/cma.j.issn.1004-4477.2015.05.021
Abstract
ObjectiveTo prepare Integrin αvβ3/CCR2 dual targeted microbubble, test physical-chemical properties, enhancement effect and targeting ability in vitro.
MethodsThe dual targeted microbubbles (MBdual-target) with FITC labled iRGD and PE labled CCR2 were prapared, and non-target microbubbles as control (MBcontrol) were prapered. Physical and chemical properties of two groups of microbubbles were tested, connectivity of peptides/antibodies and microbubbles were detected by fluorescence microscope and flow cytometry instrument.Enhancement effect and the stability of two groups of microbubbles was observed and compared in vitro. The affinity of MBdual-target and MBcontrol for bEnd.3 cells was investigated with light and fluorescent microscope.
Results①The particle size of MBdual-target was (0.93±0.23)μm, with no statistically significant difference compared with MBcontrol (P>0.05). ②MBcontrol showed no fluorescent, while MBdual-target showed both clear green and red light, under fluorescent microscope. ③There was no significant difference of gray scale of enhancement between MBdual-target and MBcontrol in vitro. ④ It was showed that MBdual-target adhered to bEnd.3 cells in vitro experiment.
ConclusionsIntegrin αvβ3 /CCR2 dual targeted targeted microbubbles was successfully prepared and proved having good enhancement effect and targeting ability in vitro.
Key words:
Microbubbles; Moleculat targeted therapy; Integrin αvβ3; iRGD; Dual-targeted
Contributor Information
Yingying Liu
Department of Ultrasonography, Shenzhen People's Hospital, Jinan University, Shenzhen 518020, China
Jinfeng Xu
Fei Yan
Hui Luo
Xinxin Zeng
Zhanghong Wei
Huiyu Liu
Yuli Zhou
