Experimental Research
RGD peptide-modified phase-change nanoparticles combining with low-intensity focused ultrasound for targeting and imaging experiments of gastric cancer in vivo and vitro
Zhangluxi Liu, Yaxu Wang, Lan Hao, Shiji Zhou
Published 2019-02-25
Cite as Chin J Ultrasonogr, 2019, 28(2): 173-179. DOI: 10.3760/cma.j.issn.1004-4477.2019.02.016
Abstract
ObjectiveTo prepare RGD peptide modified perfluorohexane (PFH) polymer nanoparticles RGD-PFH-NPs and investigate its basic characteristics, targeting ability and combine with low-intensity focused ultrasound (LIFU) for ultrasonic imaging in vivo and vitro.
MethodsTargeted nanoparticles RGD-PFH-NPs were prepared by double emulsifying method and carbodiimide method. Their morphology and distribution were observed. The particle size, zeta potential and connection probability were measured. The phase-changed properties and the LIFU-induced imaging ability in contrast-enhanced ultrasound mode of RGD-PFH-NPs were investigated in vivo and vitro. The tagetability of nanoparticles to human gastric cancer cell line MGC803 and tumor-bearing nude mouse were observed through targeting group and non-targeting group.
ResultsThe prepared sample was milky white suspension liquid. The RGD-PFH-NPs were spherical uniform size, good dispersion when observed through the optical microscope and transmission electron microscopy. The particle size was (259.3±42.6)nm and the Zeta potential was (-17.6+ 5.4)mV. The connection probability of RGD peptide was 89.13%. With 70℃ water bath and LIFU stimulation RGD-PFH-NPs can remarkably change phase and show good imaging performance in both conventional ultrasound and contrast-enhanced ultrasound mode in vivo and vitro. The connection probability to MGC803 cells in targeting group (RGD-PFH-NPs ) and the non-targeting group(PFH-NPs) were 82.59% and 2.96%. The accumulation of nanoparticles in the RGD-PFH-NPs group in tumor tissues was significantly higher than that in the non-targeted PFH-NPs group(P).
ConclusionsThe constructed nanoparticles RGD-PFH-NPs, providing contrast-enhanced ultrasonic imaging and excellent targeting ability to human gastric cancer cells MGC803 and gastric cancer tissue, is expected to become a new type of gastric cancer targeted ultrasound contrast agent.
Key words:
Contrast-enhanced ultrasound; Gastric cancer; Nnanoparticle; Phase change; Poly(lactic-co-glycolic acid)
Contributor Information
Zhangluxi Liu
Department of Gastrointstinal Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Yaxu Wang
Department of Gastrointstinal Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Lan Hao
Institute of Ultrasound Imaging, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Shiji Zhou
Department of Gastrointstinal Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
