Objective To review the epidemiology characteristic and bacterial drug resistance of nosocomial infection in the intensive care unit.Methods The distribution and drug resistance of pathogenic bacteria isolated from August 2006 to August 2008 in the intensive care unit were retrospectively analyzed.Results 214 patients(23.86 %) suffered from nosocomial infection.Among 493isolated strains, the main pathogenic bacteria were gram negative bacilli (58.01%).The proportion of Acinetobacter baumannii,Pseudomonas aeruginosa and Klebsiella pneumoniae were 19.27 %, 8.92 % and 6.90 %, respectively.In gram positive cocci(38.54%), Staphylococcus aureus accounted for 12.58 %.The rate of fungi infection was 3.45%.Conclusions Gram negative bacilli, especially the non-zymophyte,is still the main pathogenic bacteria in the intensive care unit.Moreover, the pathogenic bacteria are multidrug-resistant.Therefore, it is more important to administrate antibiotic rationally, fulfill etiology detection as soon as possible, decrease high risk factors ,and establish effective prevention and treatment.