To investigate the effects of adenovirus 5 latent infection on the oxidantantioxidant imbalance caused by exposure to biomass fumes in guinea pigs.

Forty-six albino guinea pigs were randomly divided into two groups.One group(n= 26) was infected intranasally with adenovirus 5,and the other group(n= 20)was shame-infected with sterile PBSas control.After 22 days, the living animals(20 each group)were randomly divided into two subgroups.One subgroup was exposed to biomass fumes for three weeks,and the other subgroup was exposed to room air.At the end of the experiment,animals were sacrificed and lung tissues were examined with histopathology.The persistence of the adenovirus latent infection was confirmed at the end of the study by immunohistochemistry to demonstrate the adenovirus E1A protein in the lungs of infected animals.The activities of glutathione peroxidase(GSH-PX),superoxide dismutase(SOD),and catalase(CAT),the levels of glutathione (GSH)and malondialdehyde(MDA),total ability of antioxidant in lung tissue homogenate and serum were measured.The activity of SOD,MDA and total ability of antioxidant in the bronchoalveolar lavage fluid were measured.

The latent infection of adenovirus was associated with significant decrease of GSH-PX,GSH,SOD,total ability of antioxidant in the lung tissue homogenate and serum.However,it caused decrease of CAT and increase of MDA in lung tissue homogenate.At the same time,the biomass fumes was associated with obvious decrease of GSH-PX,GSH,SOD,CAT,total ability of antioxidant in the lung tissue homogenate and serum.It was demonstrated that the biomass fumes and the latent infection of adenovirus were associated with significant decrease of GSH-PX in lung tissue homogenate and GSH-PX,GSH in serum.

Biomass fumes can lead to the oxidant-antioxidant imbalance in the lung tissue of guinea pigs,and the imbalance is amplified by the latent infection of adenovirus 5.

Smoke inhalation injury; Adenoviridae infections; Imbalance of oxidation-antioxidation; Guinea pigs