Clinical Researche
Expression and significance of CD19+ CD5+ B cells and interleukin-10 in patients with systemic lupus erythematosus
Jinhua Yang, Shaoliang Jie, Meng Wu, Chenglan Yan, Shaoran Zhang, Jing Luo, Huiling Zhang, Xiaofeng Li, Junwei Chen
Published 2015-07-15
Cite as Chin J Rheumatol, 2015, 19(7): 447-450. DOI: 10.3760/cma.j.issn.1007-7480.2015.07.004
Abstract
ObjectiveTo access the expression and clinical significance of CD19+ CD5+ B cells and interleukin (IL)-10 in patients with systemic lupus erythematosus (SLE).
MethodsForty-six SLE patients and ten healthy controls were recruited in the Second Affiliated Hospital of Shanxi Medical University. CD19+CD5+ B cells subsets were detected with flow cytometry. IL-10 level in serum were detected with enzyme linked immunosorbent assay (ELISA). The correlation between the expression of CD19+CD5+ B cells and serum level of IL-10 with ESR, systemic lupus erythematosus disease activity index (SLEDAI) score and complement was analyzed. Pair-wise comparison of means of groups was conducted with one-way ANOVA. Comparison between the two groups was conducted by LSD-t test. Correlations between variables were carried out using Spearman's rank correlation test.
ResultsThe percentage of CD19+CD5+ B cells in peripheral blood of SLE patients [(5.7± 2.1)%] was significantly lower than those in healthy control group [(19.1±2.9)%](t=2.431, P=0.005), and it had a negative correlation with SLEDAI score (r=-0.292, P=0.049). The IL-10 serum level of SLE patients [(18.8± 13.5) pg/ml] was significantly higher than the healthy control group [(8.3±2.9) pg/ml] (t=3.021, P=0.003), and it had a positive correlation with SLEDAI score (r=0.322, P=0.029).
ConclusionCD19+CD5+ B cells and IL-10 may participate in the occurrence and development of SLE. The changes of CD19+CD5+ B cells and IL-10 in the peripheral blood might contribute to the pathogenesis and correlate with the disease activity of SLE.
Key words:
Lupus erythematosus, systemic; Interleukin-10; Antigens, differentiation, B-lymphocyte
Contributor Information
Jinhua Yang
Department of Rheumatology and Immunology, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China
Shaoliang Jie
Meng Wu
Chenglan Yan
Shaoran Zhang
Jing Luo
Huiling Zhang
Xiaofeng Li
Junwei Chen