Original Article
The effects of leptin on the senescence of bone marrow-derived mesenchymal stem cells, frequencies of immune cells and lupus disease in MRL/lpr mice
Chen Haifeng, Zou Yaohong, Sun Lingyun
Published 2018-08-15
Cite as Chin J Rheumatol, 2018,22(8): 516-521. DOI: 10.3760/cma.j.issn.1007-7480.2018.08.003
Abstract
ObjectiveTo explore the effects of leptin on the senescence of bone marrow-derived mesenchymal stem cells, frequencies of Treg and Th17 cells, and lupus disease in MRL/lpr mice, and to explore the pathogenesis of systemic lupus erythematosus (SLE).
MethodsLeptin (1 μg/g) or phosphate buffer saline (PBS) was injected into C57BL/6 (B6) mice, ob/ob mice and MRL/lpr mice intra-peritoneally. Urine protein was examined by Coomassie brilliant blue method. The levels of serum leptin, antinuclear antibody (ANA), anti-dsDNA antibody and immunoglobulin (Ig)G were detected by enzyme linked immunosorbent assay (ELISA). Bone marrow derived mesenchymal stem cells (MSCs) were isolated, and treated with or without leptin, then the senescence of MSCs were evaluated by SA-β-gal staining, the levels of p53 and p21 mRNA were measured by real time polymerase chain reaction (PCR), the protein levels of p53 and p21 were tested by Western blotting method. Data were analyzed with t test and ANOVA.
ResultsThe level of serum leptin was higher in MRL/lpr mice than that of B6 mice [(4.5±0.8) ng/ml vs (2.3±0.5) ng/ml, t=2.38, P<0.05]. Leptin treatment in vivo accelerated the senescence of bone marrow-derived MSCs from all B6 mice, lupus mice and ob/ob mice, manifestedas increased frequencies of SA-β-gal positive cells [(20.6±0.6)% vs (15.4±1.6)%, t=8.09, P<0.05], higher levels of mRNA and p53 and p21 protein. Leptin treatment in vivo also down-regulated the frequency of Treg cells [(2.77±0.23)% vs (5.01±0.18)% t=3.91, P<0.01], and up-regulated Th17 cells [(2.24±0.11)% vs (1.74±0.07)%, t=5.013, P<0.01]. The titer of ANA was further increased in leptin-treated MRL/lpr mice [(288±69) U/ml vs (190±90) U/ml, t=2.84, P<0.05]. The levels of serum anti-dsDNA antibody were also remarkably elevated [(12 399±1 237) U/ml vs (6 217±1 304) U/ml, t=3.44, P<0.01]. Leptin could increase the secretion of total IgG [MRL/lpr (27.2±2.9) mg/ml vs (25.0±3.3) mg/ml, t=3.07, P<0.05]. The proteinuria concentration was increased in lupus mice [(1.00±0.10) mg/ml vs (0.81±0.06) mg/ml, t=3.31, P<0.05], demonstrating that leptin accelerates lupus nephritis.
ConclusionLeptin administr-ation in vivo enhances the senescence of bone marrow derived MSCs, dysregulate of Treg/Th17 cells from MRL/lpr mice, which impaires the role of immuno-modulation, and aggravates the progress of lupus disease.
Key words:
Leptin; Lupus erythematosus, systemic; Cell senescence; Mesenchymal stem cell; Immune regulation
Contributor Information
Chen Haifeng
Department of Rheumatology and Immunology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Jiangsu 214023, China
Zou Yaohong
Sun Lingyun
