Clinical Research
Association of tumor necrosis factor receptor-associated protein 1 gene copy number variation with susceptibility and clinical characteristics of systemic lupus erythematosus
Li Susu, Xu Jianhua, Liu Shuang, Cai Jing, Liu Shengxiu, Huang Hailiang, Qian Long, Wang Chunhuai, Pan Haifeng, Pan Faming, Su Hong, Zou Yanfeng
Published 2019-02-15
Cite as Chin J Rheumatol, 2019, 23(2): 89-94. DOI: 10.3760/cma.j.issn.1007-7480.2019.02.004
Abstract
ObjectiveTo explore whether tumor necrosis factor receptor-associated protein 1 (TRAP1) gene copy number variation was associated with susceptibility and clinical characteristics of systemic lupus erythematosus (SLE).
MethodsThe study enrolled 304 SLE patients and 391 healthy controls. They were used to investigate the association between TRAP1 gene copy number variation and SLE susceptibility. Then, 304 SLE patients were divided into copy number=2 group and copy number>2 group to study the association between TRAP1 gene copy number variation and disease activity or clinical characteristics of SLE. AccuCopyTM Kit was used to detect the TRAP1 gene copy number. Data analyses were performed by SPSS 10.01 software. The suitable method was selected among t test, rank sum test and χ2 test for analysis based on the data type and distribution, univariate and multivariate logistic regression analysis were performed to investigate the associ-ation between TRAP1 gene copy number variation and susceptibility and clinical characteristics of SLE.
ResultsThe copy number variation of TRAP1 gene showed an association with the susceptibility to SLE crude OR=5.257, 95%CI (1.108, 24.937), P=0.037; the adjusted OR=5.578, 95%CI (1.172, 26.556), P=0.031]. There was no association between TRAP1 gene copy number variation and SLE disease activity index (SLEDAI) score (Z=-0.117, P=0.907). The copy number variation of TRAP1 gene had a marginal association with skin lesions in SLE [OR=0.130, 95%CI(0.016, 1.069), P=0.058], but it disappeared after adjusting for potential confounders [OR=0.288, 95%CI(0.029, 2.831), P=0.286, PBH=0.808]. There was no correlation between TRAP1 gene copy number variation and arthritis, alopecia, oral ulcers, fever, hematologic disorder, lupus nephritis as well as photosensitivity in SLE [χ2=0.751, OR=1.234, 95%CI(0.767, 1.988), P=0.386]. No multiplicative interaction was found between TRAP1 gene copy number variation and age or body mass index (BMI) [age: χ2=0.751, OR=1.234, 95%CI(0.767, 1.988), P=0.386; BMI: χ2=0.282, OR=1.172, 95%CI(0.652, 2.109), P=0.596].
ConclusionsThe copy number variation of TRAP1 gene may be associated with susceptibility to SLE. Increased TRAP1 gene copy number may be a risk factor for SLE.
Key words:
Receptors, tumor necrosis factor, type Ⅰ; Lupus erythematosus, systemic; Variations; Disease, susceptibility; Interaction
Contributor Information
Li Susu
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, the Key Laboratory of Major Autoimmune Diseases, Hefei 230032, China
Xu Jianhua
Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
Liu Shuang
Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
Cai Jing
Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
Liu Shengxiu
Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
Huang Hailiang
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Anhui Medical University, Hefei, 230032, Anhui, China
Qian Long
Department of Rheumatology and Immunology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China
Wang Chunhuai
Department of Rheumatology and Immunology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China
Pan Haifeng
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, the Key Laboratory of Major Autoimmune Diseases, Hefei 230032, China
Pan Faming
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, the Key Laboratory of Major Autoimmune Diseases, Hefei 230032, China
Su Hong
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, the Key Laboratory of Major Autoimmune Diseases, Hefei 230032, China
Zou Yanfeng
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, the Key Laboratory of Major Autoimmune Diseases, Hefei 230032, China
