Objective To evaluate the clinical value of anterior segment optical coherence tomography (AS=OCT) on measuring the biometric structures of anterior segments of eyes in normal subjects.Methods One hundred and five eyes of 105 normal subjects were enrolled,whose biometric structures of anterior segments were measured using AS-OCT.The measuring items included central cornea thickness(CCT).anterior chamber depth (ACD),lens thickness (LT) and anterior chamber horizontal diameter(ACHD). The differences of biometric structures of anterior segments between genders were compared.The effect of age on the biometric structures of anterior segments was analyzed using linear correlation and regression.Results The mean value of CCT,ACD,LT and ACHD were(525.16±30.58)μm,(2.97±0.32)mm,(4.21±0.43) mm and(11.68±0.35)mm.respectively.The mean ACD of females was(2.87±0.33)mm,which was significantly shallower than that of males(3.07±0.29)mm (P=0.001).The CCT was (525.32±31.21)μm in females and(525.00±30.23)μm in males(P=0.957),LT was(4.26±0.42)mm in females and(4.16±0.43)mm in males(P=0.250),and ACHD was(11.64±0.32)mm in females and(1.73±0.38)mm in males(P=0.217),which showed no significant difference between genders.Age had no significant effect on CCT(r=-0.067,P=0.514)and ACHD(r=-0.137,P=0.179).There were significant negative correlation between age and ACD (r=-0.570,P=0.000),and positive relationship between age and LT(r=-0.806,P=0.000).The analysis showed decreases of ACD with age by 10(m per year,and the increase of LT with age by 19(m per year,respectively.Conclusion CCT,ACD,LT and ACHD of normal subjects can be measured quantitatively and accurately by AS-OCT. The biometric structures of anterior segments of females were shallower than that of males.With age,ACD become shallower,while LT increase in normal subjects.