Objective To investigate the clinical significance of the objective status of strabismus before and after surgery using the iris localization and discuss its effect on strabismus surgery.Methods The collection received patients in hospital with concomitant exotropia and superior oblique palsy.Using the Wave Scan wavefront analyzer was to take iris images and data,and the use of VISX Star S4-IR excimer laser,as the U.S company,were recorded iris (eye) rotation angle before and after surgery.Results The concomitant exotropia group Non-microsurgery group:The dominant eye's rotation before and after surgery was (4.88±2.55)° and (4.76±2.62)° respectively.The difference between the two groups was not significant.The main strabismus eye's rotation before and after surgery was (2.70±2.36)° and (6.00±2.76)° respectively.The difference between the two groups was statistically significant.Micro-surgery group:The dominant eye's rotation before and after surgery was (2.86±2.28)° and (3.12±2.17)° respectively.The difference between the two groups was not significant.The main strabismus eye's rotation before and after surgery was (2.08±1.86)° and (3.28±2.04)° respectively.The difference between the two groups was statistically significant.The superior oblique palsy group Non-microsurgery group:The dominant eye's rotation before and after surgery was (2.88±3.58)° and (2.08±2.36)° respectively.The difference between the two groups was not significant.The main strabismus eye's rotation before and after surgery was (2.48±2.51)°and (5.73±1.98)° respectively.The difference between the two groups was statistically significant.Micro-surgery group:The dominant eye's rotation before and after surgery was (4.90±3.60)° and (4.56±1.12)° respectively.The difference between the two groups was not significant.The main strabismus eye's rotation before and after surgery was (3.12±3.07)° and (4.26±1.98)° respectively.The difference between the two groups was statistically significant.Conclusions Micro-surgery can improve the fineness of strabismus surgery.Iris positioning technology can be an objective description of rotating the eye in patients with strabismus,in order to make a clinical evaluation of surgical results.