Original Article
MicroRNA- target gene and microRNA- small molecule interaction network analysis of fibroblast like synoviocytes in rheumatoid arthritis
De Jizhuoga, Da Wazhaba, Li Jingxiang, He Yuheng, La Baciren, Chun Hua, Li Caolong, Wang Hui, Jia Xiuzhi
Published 2017-11-05
Cite as Int J Immunol, 2017,40(6): 600-603. DOI: 10.3760/cma.j.issn.1673-4394.2017.06.003
Abstract
ObjectiveTo establish Rheumatoid Arthritis Fibroblast Like Synoviocytes(RA-FLS) background specific microRNA-target gene and microRNA-small molecule interaction network.
MethodsmicroRNA PCR Array data(GSE72564) was used to detect differential expression of microRNA between RA-FLS and osteoarthritis FLS.MicroRNA-target gene interaction data(miRTarBase, TarBase, miRecords, TargetScan) were integrated to construct the regulatory networks between the specific expression of microRNA and the target gene associated with RA-FLS.Then, microRNA-small molecule interaction data(SM2miR, Pharmaco-miR) were integrated to construct microRNA-small molecule regulatory network, and network centrality was calculated and core regulatory microRNA and small molecule nodes were obtained.
Results63 differentially expressed microRNA were got and RA-FLS background specific microRNA-target gene and microRNA-small molecule interaction network were established, then five key microRNA(hsa-let-7b-5p, hsa-mir-15b-5p, hsa-mir-218-5p, hsa-mir-30a-5p, hsa-mir-520d-3p) and four active small molecules (5-fluorouracil, glucose, 1, 2, 6-Tri-O-galloyl-beta-D-glucopyranose, glucocorticoid) were screened.
ConclusionsRA-FLS background specific microRNA-target gene and microRNA-small molecule interaction network, especially key microRNA and small molecule screening, is expected to become a potential target for RA-FLS research.
Key words:
Rheumatoid arthritis fibroblast like synoviocytes; MicroRNA; Target genes; Small molecules
Contributor Information
De Jizhuoga
Tibet College of Tibetan Medicine, Lhasa 850000, China
Da Wazhaba
Tibet College of Tibetan Medicine, Lhasa 850000, China
Li Jingxiang
Tibet College of Tibetan Medicine, Lhasa 850000, China
He Yuheng
Tibet College of Tibetan Medicine, Lhasa 850000, China
La Baciren
Tibet College of Tibetan Medicine, Lhasa 850000, China
Chun Hua
Tibet College of Tibetan Medicine, Lhasa 850000, China
Li Caolong
Tibet College of Tibetan Medicine, Lhasa 850000, China
Wang Hui
First Affiliated Hospital, Harbin Medical University, Harbin 150001, China
Jia Xiuzhi
Tibet College of Tibetan Medicine, Lhasa 850000, China
