Laboratory Research
Coxsackievirus B causes myocardial injury through inducing pyroptosis
Shi Jianing, Huang Yike, Qin Ying, Fu Jun, Wang Yan, Shi Lijun, Zhong Zhaohua, Yang Youlin, Zhao Wenran
Published 2018-05-05
Cite as Int J Immunol, 2018,41(3): 256-259. DOI: 10.3760/cma.j.issn.1673-4394.2018.03.002
Abstract
ObjectiveTo observe the effect of pyroptosis in the myocardial injury caused by Coxsackievirus B (CVB), and to determine whether CVB cause myocardial inflammation through pyroptosis.
MethodsNewborn Balb/c mice at the age of 3 days were divided into 4 groups: control groups; infected with CVB type 3 (CVB3) intraperitoneally; treated with Ac-YVAD-CMK intraperitoneally once at the same time of viral inoculation, or three times at 1, 3, 5 days after CVB3 infected.The expression level of caspase-1 protein was determined by Western blotting.The myocardial injury of the treated mice was observed with HE section.HeLa cells were divided into eight groups: four groups were infected with CVB3 for various time periods while the other four groups were infected with CVB3 and treated with various doses of caspase-1 inhibitor.
ResultsCVB3 infection induced caspase-1 activation in cardiac muscle.Compared with the mice infected with CVB3 alone, the treatment of caspase-1 inhibitor markedly reduced the level of cleaved caspase-1.Caspase-1 inhibitor also suppressed CVB3 replication as shown in Hela cells.The myocardium of the mice treated with caspase-1 inhibitor after CVB3 infection showed slightly inflammation and necrosis compared with mice infected with the virus only.
ConclusionSuppression of pyroptosis can effectively relieve themyocardial injury.The pyroptosisplays a pathological role in the CVB-related myocardial diseases.
Key words:
Coxsackievirus B; Pyroptosis; Inhibitor; Myocardial injury
Contributor Information
Shi Jianing
Department of Internal Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
Huang Yike
Department of Cell Biology, Harbin Medical University, Harbin 150081, China
Qin Ying
Department of Microbiology, Harbin Medical University, Harbin 150081 China
Fu Jun
Department of Internal Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
Wang Yan
Department of Microbiology, Harbin Medical University, Harbin 150081 China
Shi Lijun
Zhong Zhaohua
Department of Microbiology, Harbin Medical University, Harbin 150081 China
Yang Youlin
Department of Internal Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
Zhao Wenran
Department of Cell Biology, Harbin Medical University, Harbin 150081, China