Original Article
Effect of exogenous IFN-β on the induction of endogenous type I IFN in OL and OL/BDV cells
Zhai Aixia, Yan Dongmei, Wang Haiping, Cui Lele, Bu Tong, Kao Wenping, Zhang Fengmin
Published 2019-01-05
Cite as Int J Immunol, 2019,42(1): 1-6. DOI: 10.3760/cma.j.issn.1673-4394.2019.01.001
Abstract
ObjectiveTo investigate the effect of exogenous interferon (IFN) -β on the expression of endogenous type I IFN and the localization of interferon regulatory factor (IRF) 7 in human oligodendrocyte (OL) and Borna disease virus (BDV) persistently infected OL cells (OL/BDV).
MethodsThe expression of type I IFN mRNA was detected by quantitative real-time PCR (qPCR) after OL cells and OL/BDV cells were treated with exogenous IFN-β for 0, 0.5, 2, 4, 8 h and 24 h. According to whether the exogenous IFN-β was added to the cell culture, cells were divided into exogenous IFN-β unstimulated and stimulated cells.The localization of IRF7 was observed after OL cells and OL/BDV cells were transfected with IRF7-EGFP plasmids and stimulated with exogenous IFN-β for 4 h.
ResultsThe expression of IFN-α mRNA was increased when OL cells were stimulated by exogenous IFN-β for 2, 4 h, and 24 h (P values were 0.008, 0.0001, 0.004, respectively). The expression of IFN-β mRNA was increased when cells were treated for 0.5, 4, 8, 24 h(P values were 0.0004, 0.0002, 0.011, 0.012 respectively). The most significant increase for IFN-α and IFN-β mRNA were all at 4 h after treatment.The expression of IFN-α mRNA in OL/BDV cells which were treated with the same stimulation as for OL cells, increased at 4 h and 24 h(P values were 0.0004, 0.022 respectively). The expression of IFN-β mRNA increased only when treated for 4 h (P=0.002). Meanwhile, IRF7 green fluorescent protein translocated into the nucleus in OL and OL/BDV cells treated with exogenous IFN-β stimulated for 4 h.
ConclusionsExogenous IFN-β induces the expression of endogenous type I IFN in OL and OL/BDV cells.Moreover, endogenous IFN-β participates in releasing the suppression of BDV toward type I IFN, and promotes the activation of IRF7.
Key words:
Exogenous interferon -β; Human oligodendrocyte; Borna disease virus persistently infected human oligodendrocyte; Endogenous type I interferon
Contributor Information
Zhai Aixia
Department of Microbiology, Harbin Medical University Wu Lien-Teh institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity Key Laboratory of Pathogen Biology in Heilongjiang Provincial Education Institute Science and Technology Innovation Team for Infection and Immunity in Heilongjiang Provincial Education Institute, Harbin 150081, China
Yan Dongmei
The Fourth Affiliated Hospital of Harbin Medical University 150001, China
Wang Haiping
The Fourth Affiliated Hospital of Harbin Medical University 150001, China
Cui Lele
Department of Microbiology, Harbin Medical University Wu Lien-Teh institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity Key Laboratory of Pathogen Biology in Heilongjiang Provincial Education Institute Science and Technology Innovation Team for Infection and Immunity in Heilongjiang Provincial Education Institute, Harbin 150081, China
Bu Tong
Department of Microbiology, Harbin Medical University Wu Lien-Teh institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity Key Laboratory of Pathogen Biology in Heilongjiang Provincial Education Institute Science and Technology Innovation Team for Infection and Immunity in Heilongjiang Provincial Education Institute, Harbin 150081, China
Kao Wenping
Department of Microbiology, Harbin Medical University Wu Lien-Teh institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity Key Laboratory of Pathogen Biology in Heilongjiang Provincial Education Institute Science and Technology Innovation Team for Infection and Immunity in Heilongjiang Provincial Education Institute, Harbin 150081, China
Zhang Fengmin
Department of Microbiology, Harbin Medical University Wu Lien-Teh institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity Key Laboratory of Pathogen Biology in Heilongjiang Provincial Education Institute Science and Technology Innovation Team for Infection and Immunity in Heilongjiang Provincial Education Institute, Harbin 150081, China