Orginal Article
Chlorpromazine up-regulates the expression of coagulation factor Ⅲ in rat cortex
Wu Jing, Li Yang, Han Qiuyuan, Yu Li, Qian Jun, Zhang Wenli, Li Aimei, Li Yujun, Zhang Qingmeng, Song Wuqi, Kao Wenping, Pan Chao, Zhang Fengmin
Published 2017-02-15
Cite as Int J Genet, 2017,40(1): 13-18. DOI: 10.3760/cma.j.issn.1673-4386.2017.01.003
Abstract
ObjectiveTo analyze the expression of coagulation factor Ⅲ in chlorpromazine (CPZ)-treated rat cortex.
MethodsWistar rats (n=6) were assigned to normal control and CPZ treated groups. The rats of CPZ treated group were injected intraperitoneally with CPZ (5 mg/kg, 10 mg/kg and 20 mg/kg for single-injection or once-daily injection (10 mg/kg) continuously for 2 weeks. Immunohistochemistry and Western blot analysis were performed to examine the expression level of coagulation factor Ⅲ in rat cortex.
ResultsImmunohistochemistry results showed that both the single and continuous repeated use of CPZ (10 mg/kg) could lead to a significant increase in coagulation factor Ⅲ expression in rat cortex compared to control subjects (P=0.0007, 0.0005). Quantification of the Western blot indicates that both the 10 mg/kg and 20 mg/kg dose of CPZ single injection significantly increased the coagulation factor Ⅲ expression level in rat cortex (P=0.0141, 0.0163). Similarly, continuous treatment of CPZ (10 mg/kg) also resulted in a significant increase of coagulation factor Ⅲ in rat cortex compared with the untreated control group (P=0.009).
ConclusionCPZ could up-regulate the coagu-lation factor Ⅲ expression in rat cortex. Except for the dopamine receptor and calmodulince, coagulation factor Ⅲ is also a target protein of CPZ.
Key words:
Chlorpromazine; Rat; Cortex; Coagulation Factor Ⅲ
Contributor Information
Wu Jing
Wu Lien-Teh Institute, Department of Microbiology, Harbin Medical University, the Heilongjiang Key Laboratory of Immunity and Infection, Harbin 150081, China
Li Yang
Han Qiuyuan
Yu Li
Qian Jun
Zhang Wenli
Li Aimei
Li Yujun
Zhang Qingmeng
Song Wuqi
Kao Wenping
Pan Chao
Zhang Fengmin
