Original Article
An application of DNA barcoding in identification of Cricetulus Barabensis
Baobao Chen, Cuihong An, Yangxin Sun, Suoping Fan, Lixia Huo, Wen Lyu, Jianjun She
Published 2016-05-20
Cite as Chin J Endemiol, 2016, 35(5): 325-328. DOI: 10.3760/cma.j.issn.2095-4255.2016.05.004
Abstract
ObjectiveTo apply DNA barcoding technology for exploring its taxonomic status and differences in the molecular biology of Cricetulus barabensis in Shaanxi Province.
MethodsSixty-five samples of Cricetulus barabensis were collected from Dingbian, Jingbian Counties in northern of Shaanxi and Dali County in Guanzhong plain (Dingbian 58 samples, Jingbian 2 samples, and Dali 5 samples). According to the mitochondrial cytochrome C oxidase subunit Ⅰ gene (COⅠ) sequence, the genetic distance was calculated and Neighbor-Joining tree was constructed.
ResultsThe genetic distance between two samples (13.16, 13.21) and other 56 samples of Dingbian was 9.2%-10.0%. The genetic distance between the 56 samples of Dingbian and Jingbian was less than 1% and Dali was 7.2%-8.3%; the average intraspecific genetic distance of Jingbian and Dali was less than 1%. The Neighbor-Joining tree showed that all the Cricetulus barabensis samples from the three counties were separated into two large branches. The samples of 13.16, 13.21 from Dingbian together were classified into a class and the rest of the samples into another separate branch. At the same time, other samples from Dingbian except 13.16, 13.21 and Jingbian were distributed in a small branch, and Dali samples were occupied another small branch.
ConclusionUsing the DNA barcoding technology, we have determined three subspecies of Cricetulus barabensis in Shaanxi Province, Dingbian has two kinds and Dali has a different subspecies.
Key words:
Cricetulus barabensis; DNA barcoding; Mitochondrial cytochrome C oxidase subunit Ⅰ gene
Contributor Information
Baobao Chen
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Cuihong An
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Department of Medicine, Xi'an Jiao Tong University, Xi'an 710054, China
Yangxin Sun
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Suoping Fan
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Lixia Huo
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Wen Lyu
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
Jianjun She
Department of Disinfection and Vector Prevention and Control, Shaanxi Center for Disease Control and Prevention, Xi'an 710054, China
