Original Article
Correlation between blood aryl hydrocarbon receptor, cytochrome P-450 1A1 mRNA expression and skin changes in people with endemic arsenic poisoning
Na Cui, Yanhong Li, Jianping Liu, Kegong Wu, Yajuan Xia
Published 2016-09-20
Cite as Chin J Endemiol, 2016, 35(9): 645-649. DOI: 10.3760/cma.j.issn.2095-4255.2016.09.006
Abstract
ObjectiveThough measuring the expression levels of blood aryl hydrocarbon receptor (AhR) and cytochrome P-450 1A1 (CYP1A1), to explore the relationship between the expression levels and chronic arsenic poisoning induced skin changes.
MethodsTotally 233 residents were selected in Hanggin Rear Banner arsenic exposure area of Bayannur City, according to water arsenic concentrations, these people were divided into control (< 10 μg/L, 55 people), low (10-< 100 μg/L, 47), medium (100-< 200 μg/L, 45) and high (≥200 μg/L, 86) arsenic exposure groups. Real-time PCR was used to detect the expression levels of blood AhR and CYP1A1 mRNA, which were presented in median and quartile [M (Q1-Q3)], and the relationships between their expression levels and keratosis, depigmentation of skin were analyzed.
ResultsThe relative expression levels of AhR and CYP1A1 mRNA in high-dose groups were 3.18 × 10-3 (2.42 × 10-3-4.45 × 10-3) and 1.58 × 10-3 (0.80 × 10-3-2.73 × 10-3), which were higher than those in control groups [2.30 × 10-3 (1.53 × 10-3-3.20 × 10-3) and 1.00 × 10-3 (0.59 × 10-3-2.09 × 10-3)], and the difference were statistically significant (all P < 0.05). Compared with control group, the detectable rates of arsenic poisoning, keratosis and depigmentation of skin were higher, and the differences were statistically significant (χ2= 20.187, 15.848, 21.595, all P < 0.05). The detectable rates of arsenic poisoning, keratosis and depigmentation of skin were increased with increase of water arsenic concentrations (χ2 = 19.012, 15.269, 16.868, all P < 0.05). Compared with normal [2.54 × 10 -3 (1.79 × 10 -3-3.43 × 10-3 ), 2.57 × 10-3 (1.78 × 10-3-3.52 × 10-3)], AhR mRNA relative expression levels [4.45 × 10-3 (3.47 × 10-3-8.04 × 10-3), 4.45 × 10-3 (4.02 × 10-3-6.25 × 10-3)] of degree Ⅲ keratosis and depigmentation of skin were increased, and the differences were statistically significant (all P < 0.05).
ConclusionsChronic arsenic exposure affects the expression level of AhR and CYP1A1 mRNA. Blood AhR mRNA expression may have relationship with endemic arsenic poisoning induced skin change, but blood CYP1A1 mRNA expression may have nothing to do with endemic arsenic poisoning induced skin change.
Key words:
Arsenic exposure; Aryl hydrocarbon receptor; Cytochrome P450 1A1; Skin changes
Contributor Information
Na Cui
Department for Arsenic Poisoning Control and Prevention, Inner Mongolia Center for Endemic Disease Control and Research, Huhhot 010031, China
Yanhong Li
Department for Arsenic Poisoning Control and Prevention, Inner Mongolia Center for Endemic Disease Control and Research, Huhhot 010031, China
Jianping Liu
Translational Medicine Center, Baotou Central Hospital, Baotou 150200, China
Kegong Wu
Department for Arsenic Poisoning Control and Prevention, Inner Mongolia Center for Endemic Disease Control and Research, Huhhot 010031, China
Yajuan Xia
Department for Arsenic Poisoning Control and Prevention, Inner Mongolia Center for Endemic Disease Control and Research, Huhhot 010031, China
