Field Epidemiological Investigation
An observation of biochemical indexes in the fluorosis accumulation human serum in Qinghai plateau
Wei Shengying, Lu Qing, Wang Mei, Cai Shenghua, Yang Ping, Pu Guanglan, Chen Ping, Zhao Zhijin, Ma Li, Zheng Yi, He Duolong
Published 2017-02-20
Cite as Chin J Endemiol, 2017,36(02): 124-127. DOI: 10.3760/cma.j.issn.2095-4255.2017.02.011
Abstract
ObjectiveTo observe the content changes of melatonin (MT), serotonin (5-HT), xanthine oxidase (XOD), glutathione over peroxidase (GSH-Px) and calcitionin (CT) in the fluorosis accumulation human serum.
MethodsPatients with a habit of long-term drinking brick tea and clinically diagnosed skeletal fluorosis were selected in, aged 16~ 65 years, and grouped according to 10-year-old of 16-, 26-, 36-, 46-, 56-years old, each group had 50 people, half males and half females, urine samples were collected for determination of urinary fluoride content; and portable X-ray DR was used to take X-ray photographs; at the same time, their venous blood was collected to detect the content of serum MT, 5-HT, XOD, GSH-Px and CT. Fluoride content of urine was determined by "Fluoride Ion Selective Electrode in Urine" (WS/T 30-1996); X-ray diagnosis of skeletal fluorosis was based on "Diagnostic Criteria for Endemic Skeletal Fluorosis" (WS 192-2008); MT, 5-HT, XOD, GSH-Px and CT were determined with enzyme-linked immunosorbent assay (ELISA).
ResultsTotally 250 patients were checked skeletal fluorosis by X-ray, the prevalence rate of skeletal fluorosis was 73.6% (184/250), of which 88 cases were degree Ⅰ skeletal fluorosis, accounting for 35.2%; 72 cases were degree Ⅱ skeletal fluorosis, accounting for 28.8%; and 24 cases were degree Ⅲ skeletal fluorosis, accounting for 9.6%. Totally 250 urine samples were detected fluoride content, the mean was 2.13 (0.20-12.04) mg/L; of which the urine fluoride content of control group was 1.71 (0.26-4.78) mg/L; that of degree Ⅰ was 1.83 (0.20-10.00) mg/L, that of degree Ⅱ was 2.29 (0.29-8.31) mg/L; that of degree Ⅲ was 3.46 (0.51-12.04) mg/L, as the illness aggravated, there was a tendency of increased urinary fluoride (H= 22.412, P < 0.05). The mean of MT, 5-HT, XOD, GSH-Px and CT in 250 serum samples was (21.80 ± 12.48) ng/L, (362.62 ± 86.70) ng/L, (2 619.83 ± 903.90) ng/L, (51.46 ± 14.13) pmol/L and (762.91 ± 238.18) ng/L. There was a decreasing trends of MT and GSH-Px secretion (F= 197.413, 8.949, all P < 0.05) with aggravation of the disease. MT in the age groups of 16-, 26-, 36-, 46-, 56-was (36.96 ± 3.45), (29.02 ± 3.59), (24.20 ± 1.92), (10.23 ± 5.56) and (5.89 ± 4.12) ng/L, respectively. MT and GSH-Px secretion declined with age (F= 580.021, 2.456, P < 0.05).
ConclusionThe serum levels of MT and GSH-Px have increased with the severity of the disease and older of age, which needs to be further studied.
Key words:
Fluorine; Melatonin; Serotonin; Xanthine fluorinated oxidase; Glutathione over peroxidase; Calcitionin
Contributor Information
Wei Shengying
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Lu Qing
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Wang Mei
the Department of Plague, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Cai Shenghua
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Yang Ping
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Pu Guanglan
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Chen Ping
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Zhao Zhijin
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Ma Li
the Department of Brucellosis, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
Zheng Yi
the Department of Plague, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
He Duolong
The Department of Endemic Disease, Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China