Original Article
The epidemiological investigation and pathogenical analysis of human brucellosis in Tacheng and Kashgar of Xinjiang Uygur Autonomous Region
Qian Wang, Bin Yan, Zeyu Chen, Songsong Xie, Hongmei Zhang, Xiafei Liu, Dianqin Yu, Wureli Hazi, Buyun Cui, Yuanzhi Wang
Published 2019-01-20
Cite as Chin J Endemiol, 2019, 38(1): 21-24. DOI: 10.3760/cma.j.issn.2095-4255.2019.01.006
Abstract
ObjectiveTo understand the infected strains and prevalence of brucellosis in occupational population in Tacheng and Kashgar regions, Xinjiang Uygur Autonomous Region.
MethodsIn September 2015, blood samples from occupational population (including herders, semi-agricultural and semi-pastoral, veterinarians) and non-occupational population (including students and cadres) were collected to detect Brucella-specific antibody and bacterial nucleic acids by rose bengal plate test (RBPT), serological standard tube agglutination test (SAT) and PCR methods, respectively. The positive products of PCR were sent to Shanghai Sangon Biotechnology Co., LTD. Then the sequence results were retrieved online using the basic alignment search tool (BLAST) in GenBank web page and uploaded to NCBI (National Center for Biotechnology Information).
ResultsA total of 546 blood samples were tested, including 300 males, aged (55 ± 15) years old, and 246 females, aged (54 ± 12) years old. The positive rates were 17.58% (96/546) and 6.78% (37/546) in 546 blood samples by serological method and genetic markers targeting omp22 and omp2, respectively. The positive rates were statistically significant (χ2 = 29.8, P < 0.05). Additionally, based on BLAST analysis of outer membrane protein omp22 and omp2 genes, the positive products were identified as Brucella abortus, and the sequence similarity was 100.00% (253/253, 863/863 bp) to Brucella abortus strain Wisconsin genome assembly, chromosome (LT651712).
ConclusionsBrucellosis has a high infection rate in the occupational population of some animal husbandry-based groups in Xinjiang. The infection strain is abortive species Brucella, and health education for the occupational population and prevention of brucellosis should be strengthened to reduce the infection rate.
Key words:
Brucellosis; Serological; PCR
Contributor Information
Qian Wang
Clinical Laboratory, Kaifeng Central Hospital, Kaifeng 475000, China
Bin Yan
Department of Pathogenic Biology and Immunology, School of Medicine, Shihezi University, Shihezi 832000, China
Zeyu Chen
Department of Pathogenic Biology and Immunology, School of Medicine, Shihezi University, Shihezi 832000, China
Songsong Xie
Division of Infectious Diseases, the First Affiliated Hospital, Shihezi University, Shihezi 832000, China
Hongmei Zhang
Clinical Laboratory, the First Affiliated Hospital, Shihezi University, Shihezi 832000, China
Xiafei Liu
Department of Pathogenic Biology and Immunology, School of Medicine, Shihezi University, Shihezi 832000, China
Dianqin Yu
Department of Pathogenic Biology and Immunology, School of Medicine, Shihezi University, Shihezi 832000, China
Wureli Hazi
Department of Animal Genetics, Breeding and Reproduction Science, School of Animal Science and Technology, Shihezi University, Shihezi 832000, China
Buyun Cui
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Yuanzhi Wang
Department of Pathogenic Biology and Immunology, School of Medicine, Shihezi University, Shihezi 832000, China
