Original Article
Effects of excessive fluoride on osteocalcin and glucose metabolism in mice
Wen Qin, Meilin Zhang, Long Chen, Meiyu Zhang, Haiyang Ren, Jinjie Zhong
Published 2019-01-20
Cite as Chin J Endemiol, 2019, 38(1): 8-11. DOI: 10.3760/cma.j.issn.2095-4255.2019.01.003
Abstract
ObjectiveTo observe the effects of excessive fluoride on osteocalcin and glucose metabolism in mice.
MethodsThirty-two male C57 mice (body mass: 18-24 g) were selected as study subjects which were randomized into four groups (8 mice in each group) according to their body mass by random number table method: 0 mg/L group (control group), 50 mg/L fluorine group, 100 mg/L fluorine group and 150 mg/L fluorine group. Sodium fluoride in distilled water was freely taken by these animals to replicate fluorosis animal model. After 12 weeks, total osteocalcin, uncarboxylated osteocalcin, insulin and glucagon were measured by enzyme-linked immunosorbent assay, fasting blood glucose and glycated hemoglobin were measured by chemiluminescence.
ResultsAfter 12 weeks of intervention with sodium fluoride, serum total osteocalcin, uncarboxylated osteocalcin, fasting blood glucose, glycated hemoglobin, insulin, and glucagon were significantly different between different groups (F = 17.23, 22.29, 4.43, 45.57, 4.45, 55.21, P < 0.05). Total osteocalcin, uncarboxylated osteocalcin, fasting blood glucose, glycated hemoglobin, and insulin in the 100, 150 mg/L fluorine groups were higher than those of control group [(30.02 ± 5.35), (35.22 ± 4.98) vs (20.23 ± 3.22) μg/L; (8.72 ± 1.34), (11.01 ± 1.02) vs (5.80 ± 1.60) μg/L; (7.53 ± 2.29), (8.53 ± 2.81) vs (4.99 ± 1.60) mmol/L; (6.74 ± 0.68), (7.12 ± 0.25) vs (4.95 ± 0.28) mmol/L; (2.65 ± 0.25), (2.74 ± 0.47) vs (2.13 ± 0.28) mU/L, P < 0.05]. The serum glucagon levels in the 50, 100, 150 mg/L fluorine groups were lower than that in the control group [(20.90 ± 3.00), (23.68 ± 2.58), (21.63 ± 2.42) vs (38.61 ± 3.73) ng/L, P < 0.05].
ConclusionExcessive fluoride can lead to elevated osteocalcin level and abnormal glucose metabolism in mice.
Key words:
Fluorides; Osteocalcin; Glucose metabolism
Contributor Information
Wen Qin
Department of Histology and Embryology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China
Meilin Zhang
Xinjiang Urumqi Blood Center, Urumqi 830011, China
Long Chen
Functional Center, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China
Meiyu Zhang
Xinjiang Urumqi Blood Center, Urumqi 830011, China
Haiyang Ren
Clinical College, Xinjiang Medical University, Urumqi 830011, China
Jinjie Zhong
Zijingang Campus Medical School, Zhejiang University, Hangzhou 310000, China