Original Article
Molecular characterization of the Brucella ovis isolated in Nanjing
Ying Zhang, Zhifeng Zhang, Jie Hong, Weizhong Zhou, Changjun Bao, Minghao Zhou, Zhongming Tan
Published 2019-04-20
Cite as Chin J Endemiol, 2019, 38(4): 278-281. DOI: 10.3760/cma.j.issn.2095-4255.2019.04.005
Abstract
ObjectiveTo analyze the molecular characteristics of Brucella strains isolated from Nanjing, understand strains genotying and clustering, and to provide a basis for prevention and treatment of brucellosis.
MethodsMultilocus sequence typing (MLST) and multiple locus variable-number tandem repeat analysis (MLVA) were used to analyze and characterize Brucella ovis strains isolated from 7 cases of sporadic cases in Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, from 2011- 2016, and cluster analysis was did with reference strain data from Jiangsu Province.
ResultsThe results showed that 7 strains were defined as sequence type (ST) 8 by MLST. They were typed into 7 subtypes and clustered in the "Middle Mediterranean Cluster" by MLVA. Strain NJ-2011-1 and two strains isolated from other cities in Jiangsu had the same MLVA genotype.
ConclusionsThe results reveal ST8 is the predominant genotype in Nanjing. They have clustered in the "Middle Mediterranean Cluster" by MLVA. The 7 strains are sporadic. The transmission routes and risk factors are more complicated in the city. Various departments should strengthen the cooperation to control the source.
Key words:
Brucella; Multilocus sequence typing; Multiple locus variable-number tandem repeat analysis
Contributor Information
Ying Zhang
Prenatal Diagnosis Center, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Zhifeng Zhang
Laboratory, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Jie Hong
Key Lab of Enteric Pathogenic Microbiology, National Health Commission, Acute Infectious Disease Prevention Institute, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing 210009, China
Weizhong Zhou
Key Lab of Enteric Pathogenic Microbiology, National Health Commission, Acute Infectious Disease Prevention Institute, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing 210009, China
Changjun Bao
Key Lab of Enteric Pathogenic Microbiology, National Health Commission, Acute Infectious Disease Prevention Institute, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing 210009, China
Minghao Zhou
Key Lab of Enteric Pathogenic Microbiology, National Health Commission, Acute Infectious Disease Prevention Institute, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing 210009, China
Zhongming Tan
Key Lab of Enteric Pathogenic Microbiology, National Health Commission, Acute Infectious Disease Prevention Institute, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing 210009, China
