Original Article
Multilocus sequence typing characteristics of the pathogenic strain of Burkholderia pseudomallei in Hainan
Lingli Liu, Xuming Wang, Xiao Zheng, Hua Wu, Xiaojun Zhou, Hongli Guo, Kai Xu, Tianjiao Li
Published 2019-08-20
Cite as Chin J Endemiol, 2019, 38(8): 633-637. DOI: 10.3760/cma.j.issn.2095-4255.2019.08.007
Abstract
ObjectiveTo understand the pathogenicity and multilocus sequence typing (MLST) characteristics of the pathogenic strain of Burkholderia pseudomallei (Bp) in Hainan, and provide a reference for studying the structural characteristics and phylogenetic characteristics of pathogenic Bp in Hainan.
MethodsThe data of patients with melioidosis in Hainan, who were treated in Hainan General Hospital from 2011 to 2018, and Bp pathogenic strains isolated from patient infected specimens were collected. The Bp pathogenic strains were genotyped by MLST method, and the distribution characteristics of the sequence type (ST) were analyzed. The ST of all Bp pathogenic strains was analyzed by eBURST v3 software to establish the evolutionary relationship map.
ResultsA total of 91 cases of melioidosis patients were studied, including 76 males and 15 females; the ages were mainly concentrated in 40 - 70 years old; clinical manifestations included sepsis, pulmonary infection and local abscess; and the most common diseases were diabetes. A total of 91 Bp pathogenic strains were observed, of which 85 Bp pathogenic strains were distributed in coastal areas of Hainan, accounting for 93.41%; identified by MLST method, 39 ST were discovered, the most common were ST46 (13 strains), ST55 (12 strains), ST50 (8 strains) and ST58 (7 strains), accounting for 14.29%, 13.19%, 8.79% and 7.69%, respectively; except ST46 was widely distributed in the coastal areas of Hainan, the ST55, ST50 and ST58 were concentrated in the southwest, northeast and southeast regions of Hainan. Compared with the MLST database, ST30 (3 strains) was currently found only in Hainan, ST562 (4 strains) had been reported in northern Australia, and the remaining ST models had been reported in southeast Asia. The eBURST v3 software divided the 39 ST into 3 subtypes and 18 individual types. Among them, the subtypes with ST300 as the original type had the most number of ST, including 17 ST, 57 Bp pathogenic strains; compared with the MLST database, the ST300 was mainly distributed in southeast Asian regions such as Thailand.
ConclusionsThe ST of Bp pathogenic strains in Hainan has high regional diversity and genetic diversity, and is closely related to Bp in southeast Asian regions such as Thailand.
Key words:
Burkholderia pseudomallei; Multilocus sequence typing; Fungus structure
Contributor Information
Lingli Liu
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Xuming Wang
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Xiao Zheng
State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Hua Wu
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Xiaojun Zhou
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Hongli Guo
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Kai Xu
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
Tianjiao Li
Department of Clinical Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation Medicine, Hainan General Hospital, Haikou 570311, China
