Effects of liraglutide on glucose-lipid metabolism in ApoE-/-mice with RNAi-mediated adiponectin gene inhibition
ZHANG Zhi-hong, YANG Gang-yi, LIU Rui, LI Ke, LI Sheng-bin, CHEN Wen-wen, ZHU Wei
Published 2010-10-25
Cite as Chin J Endocrinol Metab, 2010,26(10): 871-874. DOI: 10.3760/cma.j.issn.1000-6699.2010.10.012
Abstract
Objective To investigate the effects of liraglutide on glucose-lipid metabolism in ApoE-/-mice with RNAi-mediated adiponectin gene inhibition. Methods The dose-effective relationship of liraglutide was evaluated by intravenous glucose tolerance test (IVGTT), and the insulin sensitivity and glucose-lipid metabolism were assessed by the hyperinsulinemic-euglycemic clamp technique using 3-[3 H]-glucose as a tracer. Results In the IVGTT, blood glucose was significantly lower in the 1 mg/kg liraglutide group than that in other groups ( all P<0. 01 ) at the points of 5, 15, and 30 min after glucose load. However, plasma insulin was significantly higher at the points of 5 and 15 min (all P<0. 01 ). Fasting blood glucose (FBG), body weight, free fatty acids (FFA),total cholesterol, triglycerides, low-density lipoprotein-cholesterol ( LDL-C), and fasting plasma insulin in ApoE-/-mice with co-injection of liraglutide and adiponectin shRNA adenovirus ( HEA group ) were significantly lower than those in ApoE-/-mice with adiponectin shRNA adenovirus injection ( ADI group, P<0. 05 or P<0. 01 ). However,high-density lipoprotein-cholesterol (HDL-C) was significantly higher than the latter (P<0. 05 ). During the steady-state of clamp, plasma insulin in ADI group was significantly higher than that in HEA group (P<0. 01 ). Although FFA, total cholesterol, and triglycerides were suppressed in all groups, they were still higher in ADI group than those in HEA group (P<0. 05). Glucose infusion rate (GIR) in HEA group were significantly higher than that in ADI group ( P < 0. 01 ). At the end of clamp, glucose disappearance rate ( GRd ) was significantly lower, and hepatic glucose production significantly higher in ADI group than those in HEA group (P<0.01 ). Conclusion Administration of liraglutide may ameliorate insulin resistance via increasing plasma adiponectin level in ApoE-/-mice with RNAi-mediated adiponectin gene inhibition.
Key words:
Liraglutide; RNA interfering; Adiponectin; Insulin resistance; Glucose-lipid metabolism
Contributor Information
ZHANG Zhi-hong
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
YANG Gang-yi
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
LIU Rui
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
LI Ke
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
LI Sheng-bin
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
CHEN Wen-wen
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
ZHU Wei
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China