Adrenal Gland
Application of SDHB and SDHC immunohistochemistry in the differentiation of malignant and benign pheochromocytoma and paraganglioma
Chuan Shi, Zhengpei Zeng, Dachun Zhao, Hanzhong Li, Qi Miao, Wenling Zhu, Shi Chen, Lin Lu, Zhiyong Liang
Published 2018-06-25
Cite as Chin J Endocrinol Metab, 2018, 34(6): 472-478. DOI: 10.3760/cma.j.issn.1000-6699.2018.06.005
Abstract
ObjectiveTo evaluate the expression profile of succinate dehydrogenase (SDH)B and SDHC in pheochromocytoma (PCC) and paraganglioma(PGL) (collectively abbreviated as PPGL), and their value in the early diagnosis of malignancy.
MethodsSDHB and SDHC immunohistochemistry were performed on 140 tumor specimens from 126 PPGL patients (PCC n=62, PGL n=61, PCC+ PGL n=3).
Results(1) Germline mutation status of 67 patients were determined, of which, identifying 37(55.2%) patients with germline mutation: 2 (3.0%) SDHA, 18 (26.9%) SDHB, 2 (3.0%) SDHC, 5 (7.5%) SDHD, 2 (3.0%)VHL, 7 (10.4%) RET, and 1(1.5%) NF1; and 30 (44.8%) individuals without known mutation. (2) Among 30 PPGLs from 27 patients with SDH-related (SDHx) mutations, 96.7% (29/30) stained negative for SDHB, 76.7% (23/30) stained negative for SDHC, while only 28.6% (14/49) and 18.4% (9/49) stained negative for SDHB and SDHC respectively in the 49 PPGLs without SDHx mutation (P<0.05). (3) The sensitivity of the SDH immunostaining in detecting the presence of germline SDHx mutation was 96.7% for SDHB and 76.7% for SDHC, while the specificity was 71.4% for SDHB and 81.6% for SDHC. (4) Among PPGLs without SDHB expression, 22.9% were malignant. This percentage is significantly higher than that in PPGLs with preserved SDHB expression (3.8%, P<0.05).
ConclusionSDHB and SDHC immunohistochemistry may serve as post-surgical screening tools to predict the presence of germline SDHx mutation in PPGLs. Negative SDHB expression calls for intense follow-up to rule out malignancy. (Chin J Endocrinol Metab, 2018, 34: 472-478)
Key words:
Succinate dehydrogenase; Immunohistochemistry; Pheochromocytoma; Paraganglioma
Contributor Information
Chuan Shi
Department of Internal Medicine, Peking Union Medical College Hospital, Beijing 100730, China
Zhengpei Zeng
Dachun Zhao
Hanzhong Li
Qi Miao
Wenling Zhu
Shi Chen
Lin Lu
Zhiyong Liang