Thyroid Cancer
Diagnostic value of pyruvate kinase M2 gene for papillary thyroid carcinoma in fine-needle aspiration specimens
Xingyue Cao, Ya Wu, Ting Xu, Hongyan Deng, Jing Hang, Jianxiang Wang, Xiao Li, Qing Yao, Rong Rong, Meiping Shen, Haixia Guan, Xiaohong Wu
Published 2019-04-25
Cite as Chin J Endocrinol Metab, 2019, 35(4): 276-281. DOI: 10.3760/cma.j.issn.1000-6699.2019.04.002
Abstract
ObjectiveTo investigate the diagnostic value of pyruvate kinase M2(PKM2)gene expression in papillary thyroid carcinoma(PTC).
MethodsQuantitative real-time PCR(RT-qPCR)was used to detect the expression of PKM2 mRNA in benign thyroid nodules, PTC, and normal thyroid tissues around nodules of fine-needle aspiration(FNA)specimens. Immunohistochemistry(IHC)was used to detect the expression of PKM2 protein in thyroid tissue after thyroidectomy. The receiver operating characteristic curve was constructed to evaluate the diagnostic value of PKM2 in PTC.
ResultsThe expression of PKM2 mRNA was detectable in FNA specimens of thyroid nodules, higher in PTC than those in normal thyroid tissue and benign thyroid nodules(P<0.01). PKM2 expression level was correlated with diameter of PTC(P<0.05), but had no correlation with lymph node metastasis, BRAFV600E mutation, and American Joint Committe on Cancer(AJCC) stage(P>0.05). The expression level of PKM2 mRNA in FNA specimens of thyroid nodules was paralleled with the expression level of PKM2 protein in postoperation pathological tissues. The accuracy, sensitivity, specificity of PKM2 gene in the diagnosis of PTC were 62.8%, 46.9%, and 95.7%, respectively. The accuracy and sensitivity of PKM2 combined with BRAFV600E were increased to 87.6% and 83.7%, significantly higher than those of single detection (P<0.05) .
ConclusionDetection of PKM2 gene in FNA specimens is highly specific in the diagnosis of PTC, making it a valuable molecular marker for preoperative diagnosis. The combination of PKM2 and BRAFV600E detection shows a higher diagnosis efficiency.
Key words:
Papillary thyroid carcinoma; Pyruvate kinase M2; Fine-needle aspiration
Contributor Information
Xingyue Cao
Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Ya Wu
Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Ting Xu
Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Hongyan Deng
Department of Ultrasound, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Jing Hang
Department of Ultrasound, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Jianxiang Wang
Department of Ultrasound, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Xiao Li
Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Qing Yao
Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Rong Rong
Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Meiping Shen
Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Haixia Guan
Department of Endocrinology, the First Affiliated Hospital of China Medical University, Shengyang 110001, China
Xiaohong Wu
Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
