Basic Research
NMDA-induced increases in intracellular Ca2+ concentrations in osteoblasts
Yanqing Liu, Lin Zhao, Shengtian Li, Jianmin Liu
Published 2019-08-25
Cite as Chin J Endocrinol Metab, 2019, 35(8): 703-710. DOI: 10.3760/cma.j.issn.1000-6699.2019.08.010
Abstract
ObjectiveTo study the effect of N-Methyl-D-asparticacid (NMDA) on the intracellular free calcium concentration ([Ca2+ ]i) in primary cultured rat calvaria osteoblasts.
MethodsA calcium imaging technique was applied to observe [Ca2+ ]i changes in primary cultured rat calvaria osteoblasts after stimulating by NMDA with various concentrations or pretreated with NMDA receptor noncompetitive antagonism MK801 (Dizocilpin).
ResultsDifferent concentrations of NMDA caused [Ca2+ ]i increases in varying degrees and by different ways. NMDA could evoke transient increase and secondary change in [Ca2+ ]i including calcium oscillation or steady increase. MK801 inhibited NMDA-induced [Ca2+ ]i increase in varying degrees.
ConclusionThese results indicated that there are abundant functional NMDA receptors expressed in primary cultured rat calvaria osteoblasts, showing different forms and varying degrees of [Ca2+ ]i increases in response to different concentrations of NMDA. The characters of the blocking effect of MK801 to NMDA-induced [Ca2+ ]i increasing, indicated that the NMDA receptors expressed in primary cultured rat calvaria osteoblasts differ in channel properties from those in central nervous system.
Key words:
Osteoblasts; NMDA receptors; Calcium oscillation
Contributor Information
Yanqing Liu
Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China (is working on the Department of Endocrinology, Shanghai Putuo District People′s Hospital, Shanghai 200060, China)
Lin Zhao
Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
Shengtian Li
School of Life Sciences &
Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China
Jianmin Liu
Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
