Diagnostic value of S100A6 mRNA expression level in endoscopic ultrasonography guided fine needle aspiration specimens in pancreatic cancer
ZHANG Jie, GUO Zi-hao, LIANG Yan, LI Xue, CHEN Jing, ZHANG Jing, ZHU Jing, GAO Ru, ZHOU Wei-zhen, HAO Jian-yu
Published 2012-05-15
Cite as Chin J Dig, 2012,32(05): 320-324. DOI: 10.3760/cma.j.issn.0254-1432.2012.05.010
Abstract
Objective To investigate the feasibitity of detecting S100A6 expression at mRNA level in endoscopic ultrasonography guided fine needle aspiration (EUS-FNA) pancreatic ductal adenocarcinoma (PDA) specimens and its diagnostic value in PDA.Methods A total of 18 PDA specimens and 22 normal pancreatic specimens were collected. RNA was extracted for reverse transcription.The expression of S100A6 gene was examined by real-time polymerase chain reaction.The cut-off value of S100A6 expression at mRNA level in PDA diagnosis was established through receiver operating characteristic (ROC) analysis. 28 patients with pancreatic head masses were selected for EUS-FNA examination,and the value of S100A6 mRNA expression level in PDA diagnosis was prospectively evaluated. The expression of S100A6 protein in PDA tissue was determined by immunohistochemistry staining.Results S100A6 mRNA expression in EUS-FNA and surgical PDA specimens (0.05023±0.10120,0.02083 ± 0.02848) was significantly higher than that of normal pancreatic tissues (0.00164±0.00202),both P<0.01.The expression of S100A6 in 22 EUS-FNA PDA specimens was significantly higher than that of 6 pancreatic benign disease biopsy specimens (0.00193 ± 0.00278,P =0.0009). There was no significant difference in S100A6 expression between 6 pancreatic benign disease biopsy specimens and normal surgical pancreatic samples (P=0.6143).When S100A6 mRNA expression in EUS-FNA specimens over 0.00525 was taken as positive diagnostic value,the sensitivity,specificity and accuracy in prospective pancreatic cancer diagnosis were 90.01%,100 % and 92.85 %,respectively.Conclusion The high expression of S100A6 mRNA in EUS-FNA specimens of PDA has good preoperative diagnostic value.
Key words:
Pancreatic neoplasms; S100 proteins; RNA, messenger; Eudosonography; Biopsy, fine-needle; Polymerase chain reaction; Gene expression
Contributor Information
ZHANG Jie
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
GUO Zi-hao
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
LIANG Yan
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
LI Xue
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
CHEN Jing
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
ZHANG Jing
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
ZHU Jing
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
GAO Ru
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
ZHOU Wei-zhen
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China
HAO Jian-yu
Department of Gastroenterology, Beijing Chaoyang Hospital affiliated to Capital Medical University, Beijing 100020, China