Objective To study the course and initial diameter of anterolateral abdominal perforators and the location of choke-vessels among these perforators,and to discuss the dissecting margins and combination options of inter-zone anterolateral abdominal perforator flaps.Methods Five fresh cadavers were perfused with gelatin-lead oxide and then scanned with CT.The scans were subject to the Materialized Interactive Medical Image Controlling System (Mimics) for 3D visualization and reconstruction.Information of the anterolateral abdominal perforators,i.e.the number,anastomosis and perfusion areas of the perforators were obtained.Another 10 embalmed adult cadaver specimens were dissected to observe the anterolateral abdominal perforators with diameter greater than 0.5 mm and measure their diameter,main course and anastomosis branches.Results There were a lot of anastomosing branches between the anterolateral abdominal perforators.The perfusion areas of the combined inter-zone perforator flaps were:(71.5 ± 9.5) cm2 for lower epigastric perforator + superficial iliac circumflex perforator flap,(140.6 ± 10.4) cm2 for superficial epigastric perforator + superficial iliac circumflex perforator flap,and (122.2± 15.2) cm2 for lower epigastric perforator + superficial epigastric perforator flap.Conclusion The number,position and course of the anterolateral abdominal perforators are constant.There are a large number of perforator anastomosis branches which make it suitable to harvest inter-zone perforator flaps with various combination.This provides a new surgical option for harvesting over-ysized flaps.