Orginal Article
Effect of hyperbaric oxygen combined with temozolomide on the apoptosis of glioma U373MG cells
Jiangong Ma, Xiaobin Wang, Xin Hou, Mingxuan Li, Shumin Fang, Yahu Liu, Cheng He
Published 2019-10-28
Cite as Chin J Naut Med & Hyperbar Med, 2019, 26(5): 404-407. DOI: 10.3760/cma.j.issn.1009-6906.2019.05.005
Abstract
ObjectiveTo investigate the effect of hyperbaric oxygen (HBO) combined with temozolomide (TMZ) on the apoptosis of glioma U373MG cells and also to explore possible mechanism involved.
MethodsUpon completion of cell culture, they were divided into 4 groups, i. e. the control group, the TMZ group, the HBO group and the HBO + TMZ group. The control group received neither HBO nor drug treatment, the TMZ group only received 50 μmol/L TMZ, the HBO group just received 0.24 MPa HBO treatment for 3 hours and the HBO + TMZ group was given 50 μmol/L TMZ 3 hours after HBO pretreatment. CCK-8 method was used to detect the effects of HBO and TMZ alone or combined use of 2 treatment methods on the proliferation of glioma U373MG cells. Flow cytometry was used to detect HBO and TMZ alone or combined use of 2 treatment methods on glioma U373MG cells and changes in the level of reactive oxygen species (ROS). Western blotting was used to detect the expression of U373MG apoptosis-related proteins.
ResultsThe proliferation inhibition of U373MG cells in the HBO+ TMZ group was more obvious as compared with that of the TMZ group(P<0.05). U373MG cells in the TMZ group showed early and late apoptosis, the rate of apoptosis in the HBO+ TMZ group was significantly higher than that of the control group [(73.19±3.58)% vs.(30.5±2.27)%] (P<0.01). As compared with the blank control and HBO groups, the expression level of Bcl-2 in the TMZ group significantly decreased, while the expression level of Bax and caspase-3 markedly increased. Furthermore, more obvious changes could be detected, when the HBO+ TMZ group was compared with the TMZ group(P<0.05 or P<0.01). ROS level in the U373MG cells of the HBO+ TMZ group obviously increased(P<0.01). With the addition of ROS scavenger NAC, apoptosis was inhibited in the HBO+ TMZ group(P<0.01).
ConclusionHBO combined with TMZ could enhance the inhibitory effect of TMZ on the proliferation of U373MG cells, and promote apoptosis induced by TMZ through up-regulating ROS levels in U373MG cells.
Key words:
Glioma; U373MG cell; Hyperbaric oxygen; Temozolomide
Contributor Information
Jiangong Ma
Department of Neurosurgery, First Affiliated Hospital of Henan University, Kaifeng 475000, China
Xiaobin Wang
Xin Hou
Mingxuan Li
Shumin Fang
Yahu Liu
Cheng He
