Original Article
The immunological evaluation of plague vaccine in cynomolgus monkeys
Dong Wei, Cheng Chen, Jinbiao Lu, Zhiyuan Wu, Yali Chang, Lei Jiao, Bingxiang Wang, Guozhi Wang
Published 2017-02-10
Cite as Int J Biologicals, 2017, 40(1): 9-12. DOI: 10.3760/cma.j.issn.1673-4211.2017.01.003
Abstract
ObjectiveTo evaluate the immune effect of a new plague vaccine composed of Yersinia pestis F1 antigen and recombinant V (rV) antigen in cynomolgus monkeys.
MethodsTwenty cynomolgus monkeys were simply randomized into 3 groups and intramuscularly injected with low-dose plague vaccine, high-dose plague vaccine and saline at 0 and 2 weeks, respectively. The serum samples were collected 2 weeks after first and second immunization, examined for total IgG antibodies by ELISA. In addition, enzyme-linked immunospot assay was used to detect IFN-γ-secreting lymphocytes in isolated peripheral blood lymphocytes. The results were compared by Student’s t-test.
ResultsNo IgG antibody was detected in the control group, but the plague vaccine induced strong antibody response. In cynomolgus monkeys immunized with low-dose or high-dose vaccine, 2 weeks after the second immunization, the geometric mean titers of anti-F1 IgG were (4.71±0.32)lg and (5.09±0.21)lg (t=-2.76, P﹤0.05), and anti-V IgG titers were (4.75±0.52)lg and (5.12±0.58)lg (t=-1.37, P﹥0.05), respectively. No significant increase of IFN-γ-secreting lymphocytes was found after in vitro stimulation with F1 and rV antigens. In cynomolgus monkeys immunized with low-dose or high-dose vaccine, when stimulated with F1, the number of IFN-γ-secreting cells was (1±1)/106 and (1±2)/106 (t=-0.16, P﹥0.05), respectively, and (7±15)/106 and (6±7)/106 (t=0.88, P﹥0.05) when stimulated with rV.
ConclusionPlague vaccine can elicit strong specific humoral response but weak cellular response in cynomolgus monkeys.
Key words:
Plague vaccine; Models, animal; Immunity, humoral; Immunity, cellular
Contributor Information
Dong Wei
Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing 100050, China
Cheng Chen
Jinbiao Lu
Zhiyuan Wu
Yali Chang
Lei Jiao
Bingxiang Wang
Guozhi Wang