To prepare super-activated platelet lysate (sPL) and study its effect on hair follicle regeneration in a mouse model of androgenetic alopecia (AGA), providing basis for AGA treatment research.

Platelet-rich plasma (PRP) was isolated from whole blood by ultrafiltration centrifugation. Concentrated platelets were frozen and thawed to obtain platelet lysate (PL). To prepare sPL, first platelet activation was performed by addition of CaCl₂ in combination with heparin, then the sample was completely lysed by freeze-thaw method and fibrinogen was depleted by temperature controlled clotting procedure. Hair follicle regeneration-related growth factors in PRP, PL and sPL were measured by ELISA. C57bl/6 AGA mouse model was established by administration of testosterone propionate. Hair follicle regeneration after treatment of sPL was revealed by hemotoxilin and eosin staining.

The amount of several hair regeneration-related growth factors in sPL obtained with ultrafiltration centrifugation and platelet activation was significantly higher than that in PRP and PL. AGA mice evidently regrowed hair after sPL treatment, and hair follicle density increased to the level of control mice (t=1.149, P=0.269 9).

Comparing with PRP and PL, sPL preparation can result in higher growth factor concentrations, and sPL has significant hair follicle regeneration effect on AGA mouse model.

Alopecia; Hair follicle regeneration; Super-activated platelet lysate