Drug susceptibility testing and randomly amplified polymorphic DNA analysis of Staphylococcus aureus from 178 children with impetigo
BIAN Cai-yun, LU Yong-hong, ZHOU Pei-mei, CHEN Ming, JIANG Cun-huo, CAI Qi, LI Xuan, ZHANG Da-wei, CHEN Qian-ming, LIU Yan, WU Bo, WANG Zhen-yuan, CHEN Na-yi, XIANG Dan-li
Published 2012-11-15
Cite as Chin J Dermatol, 2012,45(11): 767-770. DOI: 10.3760/cma.j.issn.0412-4030.2012.11.001
Abstract
Objective To test the antimicrobial susceptibility of Staphylococcus aureus from children with impetigo,and to assess the differences in randomly amplified polymorphic DNA profiles between sensitive and resistant Staphylococcus aureus strains.Methods Secretion specimens were obtained from the impetiginous lesions of 178 children,and subjected to bacterial culture.The susceptibility of 162 Staphylococcus aureus isolates against 21 antibiotics was tested.Randomly amplified polymorphic DNA PCR(RAPD-PCR)was performed to characterize the genotype of Staphylococcus aureus.Results Totally,180 bacterial strains were isolated from 178 children with impetigo in Chengdu,including 162(90.00%)Staphylococcus aureus strains.Of the 162 Staphylococcus aureus strains,148 were methicillin sensitive Staphylococcus aureus(MSSA),14 methicillin resistant Staphylococcus aureus(MRSA).The most active antibiotic was minocycline,followed by teicoplanin,quinupristin,vancomycin and nitrofurantoin,while the resistance rate to penicillin was highest,followed by that to erythromycin,clindamycin,compound sulfamethoxazole and tetracycline.All the Staphylococcus aureus isolates were sensitive to fusidic acid,nitrofurantoin,vancomycin,minocycline and teicoplanin.According to RAPD-PCR,the 162 Staphylococcus aureus strains were divided into 8 genotypes,with the three most prevalent genotypes being Ⅲ(31.48%),Ⅱ(26.54%)and Ⅵ(25.93%),which accounted for 65.43%(106/162)in all the strains.The 148 MSSA strains fell into 8 genotypes,with genotype Ⅲ(50 strains,33.78%),Ⅵ(39 strains,26.35%)and Ⅱ(33 strains,22.30%)being the most prevalent genotypes;the 14 MRSA strains fell into 3 genotypes,i.e.,genotype Ⅱ(10 strains,71.43%),Ⅵ(3 strains,21.43%),and Ⅲ(1 strain,7.14%).Conclusions Staphylococcus aureus is the most prevalent pathogenic bacteria in children with impetigo in Chengdu area,which is highly sensitive to minocycline,teicoplanin and quinupristin,and falls into 8 genotypes according to RAPD-PCR with genotype Ⅲ being the most common genotype.
Key words:
Impetigo; Staphylococcus aureus; Microbial sensitivity tests; Randomly amplified polymorphic DNA technique
Contributor Information
BIAN Cai-yun
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
LU Yong-hong
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
ZHOU Pei-mei
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
CHEN Ming
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
JIANG Cun-huo
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
CAI Qi
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
LI Xuan
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
ZHANG Da-wei
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
CHEN Qian-ming
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
LIU Yan
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
WU Bo
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
WANG Zhen-yuan
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
CHEN Na-yi
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China
XIANG Dan-li
Department of Dermatology,Chengdu City Second People's Hospital,Chengdu 610017,China