Effects of amifostine on microglial injury induced by glutamate
ZHANG Xia-jing, HE Er-tao, JIA Ji, ZHU Xiao-ling, WANG Qiang, ZHU Zheng-hua, HU Sheng, CHEN Shao-yang
Published 2012-04-15
Cite as Int J Anesth Resus, 2012,33(04): 246-250. DOI: 10.3760/cma.j.issn.1673-4378.2012.04.007
Abstract
Objective To investigate the effects of Amifostine on glutamate (Glu) induced microglia injury. Methods There are 4 groups:Con group,N9 microgial was cultured normally for 24 h,Glu group,N9 microgial was treated with 10 mmol/L Glu,Ami+Glu group,N9 microgial was treated with 1 mmol/L Amífostine and 10 mmol/L Glu,Ami+TBOA+Glu group,N9 microgial was treated with 100 μmol/L TBOA, 1 mmol/L Amifostine and 10 mmol/L Glu for 24 h.Metabolism of microglial,lactate dehydrogenase (LDH) release,superoxide dismutase (SOD) activity,nitrogen monoxide (NO) release and glutathione (GSH) level were evaluated. Results As compared to control group,microglial metabolism (88.9±1.7)% increased (P<0.05),SOD activity (128±13)% increased (P<0.01) and GSH level(88±8)% increased(P<0.01),while LDH(141±40)% and NO(147±34)% release were decreased in Amifostine group.The effects of Amifostine could be reversed by the antagonist of excitatory amino acid transporters(EAAT) TBOA. Conclusions Amifostine protects N9 microglial Glu injury through its antioxidantive effects.
Key words:
Amifostine ; Glutamate; Microglia; Excitatory amino acid transporters; Protection
Contributor Information
ZHANG Xia-jing
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
HE Er-tao
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
JIA Ji
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
ZHU Xiao-ling
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
WANG Qiang
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
ZHU Zheng-hua
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
HU Sheng
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China
CHEN Shao-yang
Deportment of Anesthesiology, Xijing Hospital, Xi'an 710032,China