Original Article
The effects of intrathecal injecting capsaicin on the analgesia effects of enflurane and sevoflurane
Zhang Mingyang, Zhou Meiyan, Dai Tijun
Published 2016-06-15
Cite as Int J Anesth Resus, 2016,37(6): 499-503. DOI: 10.3760/cma.j.issn.1673-4378.2016.06.005
Abstract
ObjectiveThe present research was designed to explore the role of transient receptor potential vanilloid 1(TRPV1) in analgesia induced by enflurane(Enf) and sevoflurane(Sev).
MethodsTwo hundred and forty mice were completely randomly divided into intraperitoneally (ip) (hot-plate test) or subcutaneously (SC) (acetic acid-induced writhing test) saline and Enf, Sev groups and each group according to intrathecal injection solvent and capsaicin (2.5, 5, 10 ng) was divided into four subgroups. After established the mice model of analgesia by intraperitoneal or subcutaneous injection of appropriate doses of Enf or Sev, we injected different doses of capsaicin intrathecally and observed effects on the pain threshold using the hot-plate and acetic acid-induced writhing test.
ResultsThe results showed that intrathecal injection of capsaicin(2.5, 5, 10 ng) did not affect the pain threshold in hot-plate test(HPPT) or the writhing numbers in conscious mice(P>0.05). In contrast, capsaicin (2.5, 5, 10 ng intrathecally) was signiflcantly decreased the HPPT and increased the writhing nubmers [Enf group: (22±7), (24±5), (29±6) vs (16±6), Sev group: (21±6), (23±7), (28±4) vs (13±5)]in mice treated with Enf or Sev(P<0.05).
ConclusionsThese results suggest that spinal TRPV1 may mediate the analgesic effects of Enf and Sev.
Key words:
Enflurane; Sevoflurane; Analgesia; Intrathecal injection; Transient receptor potential vanilloid 1
Contributor Information
Zhang Mingyang
Department of Anesthesiology, Tengzhou Central People's Hospital, Tengzhou 277500, China
Zhou Meiyan
Department of Anesthesiology, the Central Hospital of Xuzhou, Xuzhou 221009, China
Dai Tijun
Anaesthetic Pharmacology Department, Xuzhou Medical University, Xuzhou 221004, China