Original Article
Dexamethasone pretreatment can improve long-term isoflurane anesthesia induced cognitive impairment
Xueming Shao, Xin Zhao, Xiangdan Gong, Yin Cui, Zhengliang Ma, Xiaoping Gu
Published 2017-12-15
Cite as Int J Anesth Resus, 2017, 38(12): 1063-1067,1078. DOI: 10.3760/cma.j.issn.1673-4378.2017.12.002
Abstract
ObjectiveTo observe the effect of dexamethasone on cognition after long-term isoflurane anesthesia.
MethodsUsed C57BL/6 mice were randomly divided into 4 groups: control group(group C), anesthesia group(group A), dexamethasone group(group D) and dexamethasone pretreatment plus anesthesia group(group DA). Mice in group D and group DA were administrated intraperitoneally of dexamethasone at a dose of 5 mg/kg for consecutive 7 days. Mice in group A and group DA were anesthetized by isoflurane for 6 h at a concentration of 1.0%-1.5%. Space memory and contextual memory of mice were tested at day 14.
ResultsIn our study, we found that the mice in group A showed remarkable space memory impairment from day 2 to day 11(P<0.05), and dexamethasone pretreatment can reverse this effect(P<0.05). In addition, mice in group A showed more remarkable contextual memory impairment than contextual memory in group C(P<0.05) and group DA(P<0.05) at day 3. Mice in group A also showed more remarkable contextual memory impairment than memory impairment in group C(P<0.05) and group DA(P<0.05) at day 7. In addition, the relative mRNA level of N-methyl-D-aspartate receptor subunit 2B(NR2B) in group A decreased compared to group C, but dexamethasone pretreatment can prevent the decrease of NR2B mRNA from day 1 to day 7(P<0.05).
ConclusionsLong-term isoflurane anesthesia can impair space memory and contextual memory in mice which can be reversed by dexamethasone pretreatment, and glucocorticoid receptor(GR) and NR2B signal pathway may be involved in it.
Key words:
Dexamethasone; Isoflurane; Postoperative cognitive dysfunction
Contributor Information
Xueming Shao
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Xin Zhao
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Xiangdan Gong
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Yin Cui
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Zhengliang Ma
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Xiaoping Gu
Department of Anesthesiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
