Jian Zhang, Xiaobao Zhou, Qin Shi, Yuyun Sun, Yingjian Zhang, Yongping Zhang, Mingwei Wang
Abstract
ObjectiveTo develop the automated preparation of 18F-Alfatide Ⅱ using newly-designed 18F-minireactor and perform 18F-Alfatide Ⅱ microPET/CT imaging in tumor.
MethodsThe automated preparation of 18F-Alfatide Ⅱ was developed by using 18F-microreactor and water phase Al18F-chelating method, and the radiochemical yield and quality analysis were measured. The nude mice bearing breast tumor ZR-75-1 and nasopharyngeal tumor CNE1 were established (n=3 respectively). MicroPET/CT imaging was performed at 0.5, 1.0 and 2.0 h after the injection of 18F-Alfatide Ⅱ. The region of interest (ROI) was depicted and the tumor/muscle (T/M) ratio was calculated.
Results18F-Alfatide Ⅱ was automatically prepared with the total synthesis time of 40 min, the radiochemical yield of (28±6)% (no decay corrected, n=11), and the radiochemical purity >97%. All quality analysis indexes accorded with the radiopharmaceutical requirements. 18F-Alfatide Ⅱ microPET/CT images of ZR-75-1 and CNE1 tumors were clear due to the high radioactivity uptake of tumor lesions (T/M ratio was greater than 4.0 at 1.0 h after injection).
ConclusionBased on the 18F-minireactor, the 18F-Alfatide Ⅱ can be prepared successfully with short synthesis time and high radiochemical yield, which can help the application studies in 18F-Alfatide Ⅱ microPET/CT imaging.
Key words:
Peptides, cyclic; Arg-Gly-Asp; Fluorine radioisotopes; Chemistry techniques, synthetic; Breast neoplasms; Nasopharyngeal neoplasms; Neoplasm transplantation; Mice, nude
Contributor Information
Jian Zhang
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
College of Life and Environmental Sciences, Shanghai Normal University
Key Laboratory of Resource Chemistry, Ministry of Education
Shanghai Key Laboratory of Rare Earth Functional Materials
Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai 200234, China
Xiaobao Zhou
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
College of Life and Environmental Sciences, Shanghai Normal University
Key Laboratory of Resource Chemistry, Ministry of Education
Shanghai Key Laboratory of Rare Earth Functional Materials
Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai 200234, China
Qin Shi
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
Yuyun Sun
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
Yingjian Zhang
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
Yongping Zhang
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
Mingwei Wang
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center
Department of Oncology, Shanghai Medical College, Fudan University
Center for Molecular Imaging, Fudan University
Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China
