Basic Science Investigation
Biogenic gas vesicles labeled bone marrow mesenchymal stem cells: from synthesis to ultrasound imaging and tracking
Wang Yu, Yan Fei, Zhou Meijun, He Yanni, Xu Zhili, Liu Hongmei
Published 2020-06-25
Cite as Chin J Nucl Med Mol Imaging, 2020, 40(6): 362-367. DOI: 10.3760/cma.j.cn321828-20191030-00243
Abstract
ObjectiveTo fabricate biogenic gas vesicles (GVs)- polyethyleneimine (PEI)-marrow mesenchymal stem cells (BMSCs) and evaluate its potential on stem cell tracking with ultrasound imaging.
MethodsGVs were cationized by PEI to fabricate GVs-PEI. The diameter and zeta potential of GVs-PEI were determined. GVs-PEI were co-incubated with BMSCs to obtain GVs-PEI-BMSCs and stem cell uptake was observed by fluorescence microscope. The cell viability of GVs-PEI-BMSCs was verified by cell counting kit-8 (CCK-8) assay were set. Ultrasound imaging was performed on 0, 2, 4 and 6 d in agarose phantom to evaluate ultrasound imaging capability of GVs-PEI-BMSCs group and BMSCs group in vitro. GVs-PEI-BMSCs and BMSCs were injected into quadriceps femoris of SD rats, and ultrasound imaging was performed on 0, 2, 4 and 6 d to evaluate the ultrasound imaging capability in vivo. One-way analysis of variance and independent-sample t test were used to analyze the data.
ResultsThe diameter and zeta potential of GVs-PEI were (383.63±11.55) nm and (18.48±2.20) mV. Plenty of GVs-PEI were observed in GVs-PEI-BMSCs through microscope. When BMSCs were incubated with GVs-PEI in absorbance (A)500 nm of 0.5 and 1.0, there were no significant changes in the cell viability of GVs-PEI-BMSCs at 24, 48 and 72 h (F values: 7.078-11.982, all P>0.05). Compared with BMSCs, GVs-PEI-BMSCs showed better ultrasound imaging capabilityin vitro in all time points with still significantly different signal at 6 d (634.29±10.78 vs 2 864.51±100.86; t=-121.86, P<0.001). The ultrasound imaging capability of GVs-PEI-BMSCsin vivo was much better than that of BMSCs at each time point with still significantly different signal at 6 d (2 108.02±217.96 vs 267.71±7.87; t=-121.39, P<0.001).
ConclusionGVs-PEI-BMSCs are successfully fabricated with the advantages of significant ultrasound imaging capability, long duration and safety, which provide a brand-new means for stem cells tracking in vivo.
Key words:
Stem cells; Bone marrow; Gas vesicles; Polyethyleneimine; Cells, cultured; Ultrasonograhpy; Rats
Contributor Information
Wang Yu
Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, the Second School of Clinical Medicine, Southern Medical University, Guangzhou 510317, China
Yan Fei
Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Zhou Meijun
Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, the Second School of Clinical Medicine, Southern Medical University, Guangzhou 510317, China
He Yanni
Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, the Second School of Clinical Medicine, Southern Medical University, Guangzhou 510317, China
Xu Zhili
Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, the Second School of Clinical Medicine, Southern Medical University, Guangzhou 510317, China
Liu Hongmei
Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, the Second School of Clinical Medicine, Southern Medical University, Guangzhou 510317, China
