Original Article
Gene homology of novel Bunyavirus among counties in Zhoushan Islands
Li Kefeng, Li Peng, Dai Yaxin, Wang Zhongfa, Tang An, Tong Zhendong
Published 2017-08-25
Cite as Inter J Epidemionl Infect Dis, 2017,44(4): 222-227. DOI: 10.3760/cma.j.issn.1673-4149.2017.04.002
Abstract
ObjectiveTo understand gene homology of novel Bunyavirus prevailing among counties in Zhoushan Islands.
MethodsSerums in acute phase were collected from severe fever with thrombocytopenia (SFTS) patients and the viruses were cultured and isolated using Vero cell. Specific gene segments of strain (L, M and S) were amplified by RT-PCR, and sequence homology and phylogenetic analysis were carried out by software DNA Star and MEGA5.2.
ResultsThe L, M, S gene sequences of 5 SFTS virus strains could be clustered into 3 clades: the first clade complete gene sequence had relatively high homology with Korean JP series and Japanese SPL004A strains, of which the nucleotide similarity was 97.8%; the second clade had higher homology with part strains close to inland area and Japanese strain (SPL057A) , of which the nucleotide similarity was 98.8%-99.9%; and the third clade had relatively high homology with strains from Jiangsu (JS2007-1, etc.) , Liaoning (LN3/2010) , Henan (WCH-97/2011) , Anhui (AH15/2010) , Henan Huaiyangshan (2010-FQM) as well as Hubei (HB2014-20) , of which the nucleotide similarity was 97.8%-98.5%.
ConclusionsThe homology of SFTS virus prevailing in Zhoushan Island is varied. New evidence supports that SFTS virus isolates in Zhoushan Islands are probable transmitted from South Korea and other partsin mainland China.
Key words:
Bunyaviridae infections; Severe fever with thrombocytopenia syndrome; Sequence alignment
Contributor Information
Li Kefeng
Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan316021, Zhejiang, China
Li Peng
Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan316021, Zhejiang, China
Dai Yaxin
Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan316021, Zhejiang, China
Wang Zhongfa
Zhoushan Entry-exit Inspection and Quarantine Bureau, Zhoushan316021, Zhejiang, China
Tang An
Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan316021, Zhejiang, China
Tong Zhendong
Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan316021, Zhejiang, China
