Infectious Disease Research in Zhejiang·Original Article
Effectiveness of two ELISA kits for detecting IgG antibody against severe fever with thrombocytopenia syndrome virus
Shen Biao, Wang Rong, Hu Fengjiao, Ye Ling, Wang Zhongfa, Hu Xingjuan
Published 2019-04-25
Cite as Inter J Epidemionl Infect Dis, 2019,46(2): 112-116. DOI: 10.3760/cma.j.issn.1673-4149.2019.02.005
Abstract
ObjectiveTo compare two kinds of commercial ELISA kits for detecting IgG antibody against severe fever with thrombocytopenia syndrome virus (SFTSV) , so as to provide evidences for their application.
MethodsA total of 25 convalescent serum samples from confirmed cases with fever thrombocytopenia and leucopenia syndrome and 385 serum samples from healthy persons were tested by ELISA-1 kit, ELISA-2 kit and microneutralization test (MNT) . Using MNT as the gold standard test, indicators were calculated and evaluated between ELISA-1 kit and ELISA-2 kit.
ResultsThe results of 2 ELISA kits for testing IgG antibody of confirmed cases were as good as MNT. However, there were great differences among MNT, ELISA-1 and ELISA-2 for testing IgG antibody of healthy persons with the positive rates of 11.69%, 7.79% and 5.97%, respectively. Compared to MNT, ELISA-1 and ELISA-2 kits revealed the sensitivity of 51.11% and 48.89%, specificity of 97.94% and 99.71%, Youdon’s index of 0.49 and 0.49, positive likelihood ratio of 24.83 and 166.22, negative likelihood ratio of 0.50 and 0.51, consistency value of 0.57 and 0.62. S/CO ratio in false positive samples was 3.02, which was lower than that in mutual positive samples of 17.49. Seven false positive samples had the S/CO ratio of lower than 3.8.
ConclusionsELISA-1 and ELISA-2 kits are reliable for detecting IgG antibody of patients with fever thrombocytopenia and leucopenia syndrome. However, the results are not good for detecting IgG antibody of healthy persons due to low consistency and poor diagnostic value.
Key words:
Bunyaviridae; Microneutralization test; Consistency
Contributor Information
Shen Biao
Zhoushan Entry Exit Inspection and Quarantine Bureau, Zhoushan 316021, Zhejiang, China
Wang Rong
Institute of Viral Disease, Ningbo Center for Disease Control and Prevention, Ningbo 315000, Zhejiang, China
Hu Fengjiao
Institute of Viral Disease, Ningbo Center for Disease Control and Prevention, Ningbo 315000, Zhejiang, China
Ye Ling
Department of Infectious Disease Control and Prevention, Daishan Center for Disease Control and Prevention, Zhoushan 316200, Zhejiang, China
Wang Zhongfa
Zhoushan Entry Exit Inspection and Quarantine Bureau, Zhoushan 316021, Zhejiang, China
Hu Xingjuan
Zhoushan Entry Exit Inspection and Quarantine Bureau, Zhoushan 316021, Zhejiang, China
