Research, Diagnosis and Treatment of Liver Related Diseases
The mutation of HBV S gene on failure of mother-to-child transmission blocking in Yunnan Province
Kang Wenyu, He Haiyu, Li Qiongfen, Wang Feng, Shen Liping, Zhang Lifen, Xu Lin, Tian Ziying
Published 2019-12-25
Cite as Inter J Epidemionl Infect Dis, 2019,46(06): 489-494. DOI: 10.3760/cma.j.issn.1673-4149.2019.06.008
Abstract
ObjectiveTo investigate the genovariation of HBV S gene on failure of mother-to-child transmission blocking in Yunnan Province.
MethodsA total of 26 pairs of HBsAg-positive perinatal mothers and their newborns who confirmed failure of prevention of mother to child transmission (PMTCT) were randomly selected from January to June, 2011 in Yunnan. Their serum samples were collected for HBV DNA detection and gene sequencing. The further research was performed for the variation points of "a" antigenic determinant of HBV S gene in both mother group and infant group.
ResultsOf the 26 pairs of mother and infant samples who were failure of PMTCT, 9 pairs(34.62%) belonged to C genotype, and 11(42.31%) to B genotype. The rest 6 pairs(23.08%) had genetic mutations, in which 2 pairs had mutations of gene subtype belonged to C gene and 4 pairs had variations of genotype from mother to infant. There were 7 variation points in "a" antigenic determinant of HBV S gene, including T126I, T131N, M133L, F134Y, T140I,T143S and D144A. Double point mutation was the most frequent mutation in both mother(14 samples) and infant group (11 samples) . 126I/T143S point mutations of C3 genotype had the highest mutation rate. The average HBV DNA levels were>2×105 IU/mL in both mother group and infant group. The mutation rates in "a" antigenic determinant of HBV S gene were 56.75% (21/37) , 60.00% (3/5) and 100.00% (10/10) in high (>2×105 IU/mL) , middle(500-2×105 IU/mL) and low (<500 IU/mL) HBV DNA level group, respectively.
ConclusionsIn these cases with failure of PMTCT, the factor of genovariation is not dominant but should not be ignored in Yunnan. The points of mutation in "a" antigenic determinant of HBV S gene are dispersed, and have not yet been formed advantage mutants. It should be strengthen the monitoring in order to prevent the spread of the mutant strains.
Key words:
Hepatitis B; Preventing mother-to-infant transmission; Genovariation; Amino acid sequence
Contributor Information
Kang Wenyu
Institute for Immunization Program, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
He Haiyu
Department of Digestive System, the Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China
Li Qiongfen
Institute for Immunization Program, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Wang Feng
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Shen Liping
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Zhang Lifen
Institute for Immunization Program, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Xu Lin
Institute for Immunization Program, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Tian Ziying
Institute for Immunization Program, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China