Effects of icarisid Ⅱ on cognitive deficits and expression of synaptophysin in chronic cerebral hypoperfusion rat models
Liu Tao, Peng Qing, Yu Changyin, Gong Qihai, Li Guoyan
Published 2016-10-01
Cite as Chin J Prim Med Pharm, 2016,23(19): 2901-2905,C19-1
. DOI: 10.3760/cma.j.issn.1008-6706.2016.19.006
Abstract
ObjectiveTo observe the effects of icarisid Ⅱ (ICS Ⅱ) on cognitive deficits and expression of synaptophysin(SYN) in chronic cerebral hypoperfusion(CCH) rat models.
Methods40 male SD rats were randomly divided into four groups: normal group, sham operation group, model group and ICS Ⅱ group.The model was established by permanent bilateral common carotid artery occlusion(BCCAO). ICS Ⅱ group was administered ICS Ⅱ at a dose of 8mg·kg-1·d-1 by gavage on 1st day after modeling.Sham group and CCH group were injected double-distilled water.The escape latency(s) and spatial probe times were measured by water maze test.Then, the morphology change and expression of SYN in hippocampal were assayed by HE and immunohistochemistry analysis.
ResultsAt the 1st month and 2nd month, the escape latency in the model group[(40.02±4.95) s, (42.29±5.75) s]were significantly prolonged compared with the sham operation group[(26.43±2.68)s, (26.84±2.06)s](t=4.89, 5.06, all P<0.05). And those in the ICS Ⅱ group[(30.58±3.03) s, (29.19±4.23) s]were significantly decreased compared with the model group(t=3.63, 4.10, all P<0.05). The space probe times in the model group[(2.6±0.89) times, (2.40±1.14) times]were significantly reduced compared with the sham operation group[(6.00±2.16) times, (5.75±2.16) times](t=3.23, 4.18, P<0.05). And those in the ICS Ⅱ group[(4.40±1.34) times, (5.00±1.58) times]were significantly increased compared with the model group (t=2.49, 2.98, all P<0.05). The average optical density of SYN in hippocampal in the model group[CA1: (0.121±0.009), (0.122±0.008); CA3: (0.172±0.028), (0.173±0.021)]were significantly reduced compared with the sham operation group[CA1: (0.131±0.006), (0.136±0.007); CA3: (0.218±0.035), (0.204±0.018)](t=2.43, 2.53, 3.12, 2.34, P<0.05). Those in the ICS Ⅱ group[CA1: (0.131±0.006), (0.132±0.006); (0.212±0.02), (0.208±0.022)]were significantly increased compared with model group(t=2.41, 2.41, 2.31, 2.77, all P<0.05). However, there was no statistically significant difference of those index between the normal group and sham operation group(P>0.05).
ConclusionICS Ⅱ can improve the cognitive deficits in CCH rat models and this effect may be associated with increased expressions of SYN in hippocampal.
Key words:
Icarisid Ⅱ; Chronic cerebral hypoperfusion; Cognitive deficits; Synaptophysin
Contributor Information
Liu Tao
Department of Neurology Medicine, the Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, China
Peng Qing
Department of Neurology Medicine, the Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, China
Yu Changyin
Department of Neurology Medicine, the Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, China
Gong Qihai
Key Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, Guizhou 563099, China
Li Guoyan
Department of Neurology Medicine, the Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, China
