Original Article
RNA interference gene therapy targeting NHERF1 inhibits proliferation of prostate cancer cell line PC-3M
Ma Qiang, Zheng Junfang, Jiao Yanna, Gao Honglin, Liu Qiang, Liu Jianfeng, Li Deguan, Song Naling
Published 2015-01-08
Cite as J Int Oncol, 2015,42(1): 14-17. DOI: 10.3760/cma.j.issn.1673-422X.2015.01.004
Abstract
ObjectiveTo investigate the effect of NHERF1-shRNA on malignant behaviors of prostate cancer cell PC-3M.
MethodspSuper.puro NHERF1 shRNA vector and negative control (pSuper.puro luciferase shRNA) were transfected into prostate cancer cell PC-3M. Then the cell line stably knockdown NHERF1 was obtained and verified by Western blot analysis. Methyl thiazolyl thiazolium (MTT) was used to detect the proliferative activity of PC-3M cell. The cell migration abilities were examined by wound healing assay. Flow cytometry method was applied to detect the effect of NHERF1-shRNA on apoptosis of PC-3M cell line. The expressions of Bax and Bcl-2 in NHERF1-shRNA cells were detected by Western blot.
ResultsCompared with PC-3M cells and the cells which were integrated of empty vectors, NHERF1-shRNA could weaken the proliferation of PC-3M cells significantly (F=62.63, P=0.004), and significantly repress PC-3M cells migration, and increase the apoptosis percent of PC-3M cell line apparently [(2.55±0.92)% and (11.98±3.28)%], up to 4 times (t=-2.392, P=0.001). NHERF1-shRNA could reduce the expression of Bcl-2 and increase the expression of Bax.
ConclusionNHERF1-shRNA can inhibit the proliferation and migration of PC-3M cell, and promote its apoptosis.
Key words:
Prostatic neoplasms; Apoptosis; NHERF1
Contributor Information
Ma Qiang
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Science, Tianjin 300192, China
Zheng Junfang
Jiao Yanna
Gao Honglin
Liu Qiang
Liu Jianfeng
Li Deguan
Song Naling
