Basic Researches
Expression of calcium/calmodulin dependent protein kinaseⅡin spinal cord of the rats followed lidocaine hydrochloride intrathecal injection
WEN Xian-jie, XU Shi-yuan, LIANG Hua, LIU Hong-zhen, WANG Han-bing, YANG Cheng-xiang
Published 2013-01-20
Cite as Chin J Behav Med & Brain Sci, 2013,22(1): 3-5. DOI: 10.3760/cma.j.issn.1674-6554.2013.01.002
Abstract
ObjectiveTo observe the expression of calcium/calmodulin dependent protein kinaseⅡ(CaMKⅡ) in the spinal cord of the rats followed lidocaine hydrochloride intrathecal injection.
Methods48 male SD rats weight(230±20)g, after intrathecal indwelling catheter, were randomly divided into four groups (n=12, 8 rats for behavioral detection and 4 rats for western blotting): normal group (C group), sham group (S group), DMSO group (D group), 10% lidocaine group (L group). Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL) were detected before and after 2 h, 4 h, 8 h, 12 h, 1 d, 2 d, 3 d , 4 d and 5 d with drug treatment. Intumescentia lumbalis of the spinal cord were collected to measure the expression of CaMKⅡwith western blotting after drug treatment for 12 h.
ResultsThe based MWT of the rats in C, S and D group were (11.2±3.1)g, (11.8±2.2)g and (11.4±2.4)g respectively. There were no differences among the every time points (n=8, P>0.05). The MWT of the rats in L group significantly increased at 2 h, 4 h, 8 h, 12 h, 1 d , 2 d, 3 d and 4 d after treatment with lidocaine hydrochloride, and the data were (22.0±6.6)g, (22.2±5.3)g, (20.5±5.8)g, (18.5±4.3)g, (16.7±3.2)g, (15.2±3.1)g, (15.5±3.5)g , (13.7±2.4)g respectively(n=8, P<0.01). TWL had no difference among the rats in C, S, and D group(n=8, P>0.05). The TWL of the rats in L group significantly increased at 2 h, 4 h, 8 h, 12 h, 1 d, 2 d and 3 d after treatment with lidocaine hydrochloride(n=8, P<0.01). The expression of CaMKⅡ of the rats in C group, S group, D group and L group were 0.17±0.03, 0.16±0.03, 0.19±0.05, 0.42±0.11, and significantly upregulated in L goup (n=4, P<0.01).
ConclusionLidocaine hydrochloride intrathecal injection can increase the expression of the CaMKⅡin the spinal cord of the rats.Those indicate that CaMKⅡ may be involved with the nerve damage induced by lidocaine hydrochloride.
Key words:
Lidocaine hydrochloride; Spinal cord; Nerve damage; Calcium/calmodulin dependent protein kinaseⅡ
Contributor Information
WEN Xian-jie
Department of Anesthesiology, the First People's Hospital of Foshan City, Foshan 528000, China
XU Shi-yuan
LIANG Hua
LIU Hong-zhen
WANG Han-bing
YANG Cheng-xiang