Basic Research
Establishment of delay eyeblink conditioned reflex and the acquisition rule in cynomolgus monkey
Rui Li, Fang Yan, Yingshan Yang, Chuanxu Hong, Guanren Chen, Haiying Wu, Zhaoyang Tian, Jianfeng Sui, Bing Wu
Published 2016-03-20
Cite as Chin J Behav Med & Brain Sci, 2016, 25(3): 198-203. DOI: 10.3760/cma.j.issn.1674-6554.2016.03.002
Abstract
ObjectiveTo investigate the method of establishing a model of delay eyeblink conditioning (DEC) in no surgical cynomolgus monkey and analyze the related acquisition rule.
MethodsUsing the special monkey chairs to fix 6 adult male cynomolgus monkeys, they were sitting on the chairs and keeping awake, and heads could rotate freely during the whole training session. In the experiment, the 6 monkeys were trained in DEC with a tone (500 ms, 85 dB) as the conditioned stimulus (CS), paired with a corneal oxygen-puff (100 ms, 5 psi) as the unconditioned stimulus (US). There were 120 trials per-session, and 2 sessions per-day. During the experiments, an infrared emitter/detector attached to spectacles for eyeblink recording, the data of conditioned response (CR) and startle response (SR) were analyzed offline.
ResultsAmong the 6 cynomolgus monkeys, 4 of them completed all of the training process of DEC and 3 monkeys had successful acquisition of DEC. The average CR rate in the last training session reached (64.67±2.00)%, (P<0.01); 1 monkey only showed a high acquisition rate (85.00%) at the first training session, and the low CR rate in the next training sessions with the average rate of 18.16%, suggesting that the DEC model was failed to established. The SR rates in 4 cynomolgus monkeys were low with average rate of 5.47%.
ConclusionAlthough the DEC behavior training of the cynomolgus monkey is easy to be influenced by many inside or outside factors, DEC can be obtained under the condition of non surgery and proper braking.
Key words:
Delay eyeblink conditioning (DEC); Cynomolgus monkey; Acquisition rate
Contributor Information
Rui Li
Rehabilitation Department of Guizhou Provincial People’s Hospital, Guiyang 550001, China
Fang Yan
Rehabilitation Department of Guizhou Provincial People’s Hospital, Guiyang 550001, China
Yingshan Yang
Hainan Jinggang Biotech Co. Ltd., Haikou 571100, China
Chuanxu Hong
Hainan Jinggang Biotech Co. Ltd., Haikou 571100, China
Guanren Chen
Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou 570311, China
Haiying Wu
Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou 570311, China
Zhaoyang Tian
Hainan Jinggang Biotech Co. Ltd., Haikou 571100, China
Jianfeng Sui
Experimental Center of Basic Medicine, Third Military Medical University, Chongqing 400038, China
Bing Wu
Experimental Center of Basic Medicine, Third Military Medical University, Chongqing 400038, China
