：Objective To investigatethe expressions of hypoxia-inducible factors(HIF)and its related proteins inhepatoblastoma.Methods Between 1998 and 2009,30 patients who were diagnosed withhepatoblastoma and 30 patients with biliary atresia were recruited in this study.Thespecimens of the tumor group were collected from the hepatoblastoma tissue.The specimensof the first control group were the liver tissues which located more than 5 cm away fromthe borderline of the tumor.The specimens of the second control group were the livertissues from the 30 patients with biliary atresia.Immunohistochemical staining wasperformed to examine the expression level of HIF-1α,HIF-2α,vascularendothelial growth factor(VEGF),glucose transporter-1(GT1),and transforming growthfactor-alpha(TGF-α)in specimens.Results The positive stained proteinsappeared as brown granules in cytoplasm.The expression levels of all the 5 proteins of thetumor group were significantly higher than those of the other two controlgroups(P<0.01).The expression level of HIT-1αand TGF-α of the high-grade tumors were significantly higher than those ofthe low-grade tumors(P<0.05).Conclusions The expressions of HIF-1α,HIF-2α,VEGF,GT1and TGF-α increasein hepatoblastoma tissues.It indicates HIF may play a role in the carcinogenesis ofhepatoblastoma.