Kaneko's rabbit heart-lung transplant model was used to investigate the effect of low flow perfusion (LFP) on rabbit heart-lung preservation. Thirty-two healthy rabbits were randomly divided into control group and experimental group. The donors in the experimental group were continuously perfused via aorta and pulmonary artery at low flow rate with the same perfusates during preservation. After heart-lung transplanted, the donors' LVsp, LVedp, RAP and Paw, and PaO₂ were measured every 30 min for 90 min to-evaluate the function of heart and lung. After finishing the trial, the contents of MDA in heart and lung were determined and the water weight gain calculated. The results showed that the rabbits in the experimental group had a significantly lower water weight gain and better cardiac and pulmonary function than in the control group. The experimental group produced less levels of MDA in heart and lung than did the control group. It is concluded that LFP is more effective than cold storage on rabbit heart-lung preservation.