Evaluation of myocardial viability during cold storage by measurement of myocardial dielectric properties tanδm in radio frequency
DUAN Gui-xin, LIU Xue-gang, WANG Guo-wen, LIU Ge, ZHANG Lei, WANG Kang-wu, WANG An-sheng, SHI Chao
Published 2011-09-20
Cite as Chin J Organ Transplant, 2011,32(09): 558-561. DOI: 10.3760/cma.j.issn.0254-1785.2011.09.013
Abstract
Objective To study the mechanism of myocardial dielectric property changes in radio frequency during hypothermic preservation and explore myocardial viability evaluative method. Methods Hybrid young pigs (20-30 kg) were used in the experiment. Heart arrest was induced with GIK solution. According to preservative temperature, the animals were divided into three groups: group A (4 ℃),group B (15 ℃) and group C (25 C). The heart was preserved in saline for 12,6 and 4 h respectively. Myocardial dielectric properties and ATP content were tested every 5 and 30 min during hypothermic preservation respectively. The relationship between tanoδm and ATP content was analyzed. Results Tanδm of three groups was decreased significantly at the beginning, most slightly in group A and most obviously in group C. There was no significant difference in Tanδm between group B and group C with group A at 1 h,but there was obvious difference at 2,3 and 4 h (P<0. 05,P<0. 05,P<0. 01 ). There was correlation between tanδm and ATP in the three groups. Conclusion Tanδm in three groups is dropped with preservation time and temperature, and tanδm had a correlation with ATP content. Detection of myocardial dielectric property in radio frequency is a kind of quick, accurate and noninvasive method.
Key words:
Swine, Miniature; Myocardium; Cryopreservation; Dielectric property
Contributor Information
DUAN Gui-xin
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
LIU Xue-gang
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
WANG Guo-wen
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
LIU Ge
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
ZHANG Lei
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
WANG Kang-wu
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
WANG An-sheng
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
SHI Chao
Department of Cardiothoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China