A comparison study on venous anastomosis for right donor kidney transplantation in rats
DENG Xin-jun, LIU Yong-guang, GUO Ying, LI Min, YUE Liang-sheng, FAN Li-pei, CHEN Hua, ZHANG Jian-qiang, WEI Ya-fei, HE Min-yi, ZHAO Ming
Published 2012-12-20
Cite as Chin J Organ Transplant, 2012,33(12): 746-749. DOI: 10.3760/cma.j.issn.0254-1785.2012.12.012
Abstract
Objective To explore the optimized method of venous anastomosis for right donor kidney transplantation in rats.Methods Sprague Dawley (SD) rats were used as donors and recipients for homologous rat kidney transplantation.Both bilateral kidneys were harvested from the donor rats (n =45).Ninety rats were used as recipients and divided into 4 groups according to randomly digital table:In groups AC (n =15 each),the right donor kidneys were transplanted into the left nephridial pit of recipients,and endto-side,venous bypass and modified end-toend (donor's proximal end of vena cava was anastomosed to recipients renal Vein followed by ligation of its distal end) venous anastomosis was done,respectively; In the control group (n =45),the left donor kidneys were transplanted into the same side of the recipients,and the conventional end-to-end venous anastomosis was used.Then the intra-operative findings,successful operation rate and postoperative complications were compared between two groups.Results The venous anastomosis time in group B was longer than in groups A,C and control group (P<0.05),which significantly increased warm ischemia time of donor kidneys and operative time of recipients (P<0.05).The venous anastomosis time,warm ischemia time of donor kidneys and operative time of recipients showed no significant difference between groups A or C and control group (P>0.05).The successful operation rate in group C (93.3%)was similar to that in control group (86.7%) (P>0.05),but higher than in group A (53.3%) and group B (53.3%) (P<0.05).There was no significant difference in postoperative complications between group A and group C.Conclusion For right donor kidney transplantation,the method of harvesting the right donor kidney with a part of vena cava,and then anastomosing the proximal end to recipients renal vein and ligating the distal end,is highly feasible,efficient and economic.
Key words:
Kidney transplantation; Rats; Model, animal
Contributor Information
DENG Xin-jun
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
LIU Yong-guang
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
GUO Ying
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
LI Min
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
YUE Liang-sheng
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
FAN Li-pei
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
CHEN Hua
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
ZHANG Jian-qiang
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
WEI Ya-fei
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
HE Min-yi
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China
ZHAO Ming
Department of Organ Transplantation,Zhujiang Hospital, South Medical University ,Guangzhou 510282, China